Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University.
Biol Pharm Bull. 2013;36(10):1634-9. doi: 10.1248/bpb.b13-00522. Epub 2013 Aug 6.
Ginsenoside Rb1 (GRb1), one of the principle active ingredients of Panax ginseng, exerts multiple pharmacological activities to fight fatigue. In the present study, we investigate the anti-fatigue effect of GRb1 on postoperative fatigue syndrome (POFS) in a rat model induced by major small intestinal resection. GRb1 (10 mg/kg) was administrated intraperitoneally once daily for 1, 3, 7, and 10 d from the operation day. Anti-fatigue effect was assessed by grasping test and biochemical parameters in blood or skeletal muscle were determined by autoanalyzer or commercially available kits. Transmission electron microscope was applied to observe the ultra microstructure of skeletal muscles. The results revealed that GRb1 significantly enhanced rat maximum grip strength with POFS. Similarly, negative alterations in biochemical parameters (lactic acid, hepatic glycogen, muscle glycogen and malondialdehyde) of POFS rats were improved by GRb1. In addition, GRb1 also increased the activity of lactate dehydrogenase and superoxide dismutase in POFS. No significant differences of levels of blood urea nitrogen and ultra microstructure of skeletal muscles were found between the POFS and GRb1 treatment rats. The potent anti-fatigue effect of GRb1 on POFS might be achieved through improvement of energy metabolism and suppression of skeletal muscle oxidative stress.
人参皂苷 Rb1（GRb1）是人参的主要活性成分之一，具有多种药理活性，可抗疲劳。在本研究中，我们研究了 GRb1 对由主要小肠切除引起的术后疲劳综合征（POFS）大鼠模型的抗疲劳作用。从手术日起，GRb1（10mg/kg）每天腹膜内给药一次，连续 1、3、7 和 10 天。通过握力试验评估抗疲劳作用，通过自动分析仪或市售试剂盒测定血液或骨骼肌中的生化参数。应用透射电镜观察骨骼肌的超微结构。结果表明，GRb1 可显著增强 POFS 大鼠的最大握力。同样，GRb1 改善了 POFS 大鼠生化参数（乳酸、肝糖原、肌糖原和丙二醛）的负向改变。此外，GRb1 还增加了 POFS 中乳酸脱氢酶和超氧化物歧化酶的活性。POFS 和 GRb1 治疗大鼠的血尿素氮水平和骨骼肌超微结构无显著差异。GRb1 对 POFS 的有效抗疲劳作用可能是通过改善能量代谢和抑制骨骼肌氧化应激来实现的。
