Jiang Jinhui, Fan Guangxiong, Wen Rong, Liu Tao, He Shuran, Yang Shengchao, Zi Shuhui
College of Agronomy and Biotechnology, Yunnan Agricultural University (YNAU), Kunming, China.
College of Resources and Environment, Yunnan Agricultural University (YNAU), Kunming, China.
Front Microbiol. 2024 Dec 12;15:1497987. doi: 10.3389/fmicb.2024.1497987. eCollection 2024.
The biological activities of osthole have been widely reported in recent years. However, few studies have been conducted on osthole in agriculture, and its effects on plant growth have little been reported.
Three experimental treatments were set up in this experiment: blank control (CK), osthole (CLS), and (LKWS). In this study, the effects of osthole and on the growth parameters, photosynthesis, antioxidant enzyme activities, disease incidence, and microbiome of forested were tested.
This study demonstrates that the use of osthole and significantly improved the growth of in a forest compared to that in the control treatment, increased the total chlorophyll and carotenoid content of , significantly increased its net photosynthetic rate, and decreased the stomatal conductance and intercellular CO levels. In addition, the use of osthole and significantly improved ascorbate peroxidase and peroxidase (POD) activities, enhanced antioxidant activities of the POD, and reduced the disease incidence and index of American ginseng anthracnose. Based on the American ginseng microbiome analysis, the use of osthole and could change the structure of the American ginseng microbial community, significantly increase the diversity of American ginseng bacteria, significantly decrease the diversity of American ginseng fungi, stimulate the recruitment of more growth-promoting microorganisms to American ginseng, and build a more stable microbial network in American ginseng.
In conclusion, we found that the application of osthole had a positive effect on the growth of American ginseng, providing a theoretical basis for its subsequent application in agriculture.
近年来，蛇床子素的生物活性已被广泛报道。然而，关于蛇床子素在农业方面的研究较少，其对植物生长的影响鲜有报道。
本实验设置了三种实验处理：空白对照（CK）、蛇床子素（CLS）和[此处原文缺失具体内容]（LKWS）。在本研究中，测试了蛇床子素和[此处原文缺失具体内容]对林下西洋参生长参数、光合作用、抗氧化酶活性、发病率和微生物群落的影响。
本研究表明，与对照处理相比，使用蛇床子素和[此处原文缺失具体内容]显著改善了林下西洋参的生长，增加了西洋参的总叶绿素和类胡萝卜素含量，显著提高了其净光合速率，并降低了气孔导度和细胞间CO₂水平。此外，使用蛇床子素和[此处原文缺失具体内容]显著提高了抗坏血酸过氧化物酶和过氧化物酶（POD）活性，增强了西洋参POD的抗氧化活性，并降低了西洋参炭疽病的发病率和病情指数。基于西洋参微生物群落分析，使用蛇床子素和[此处原文缺失具体内容]可改变西洋参微生物群落结构，显著增加西洋参细菌的多样性，显著降低西洋参真菌的多样性，刺激更多促进生长的微生物在西洋参中定殖，并在西洋参中构建更稳定的微生物网络。
总之，我们发现蛇床子素的应用对西洋参生长有积极影响，为其后续在农业中的应用提供了理论依据。
