Dept. Bioquímica y Biología Molecular-A, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.
Biotechnol Prog. 2013 Nov-Dec;29(6):1558-64. doi: 10.1002/btpr.1791. Epub 2013 Oct 3.
The commercial value of Terfezia claveryi, an edible desert truffle with important gastronomic, nutritional, and antioxidant properties, has led to growing interest in its cultivation. The erratic and slow growth of T. claveryi mycelium in vitro represents an impairment to obtain mycorrhizal plants, and it makes necessary to find a new culture medium able to overcome these drawbacks. In this work, we analyze the effect of cyclodextrins (CDs) on the growth of T. claveryi mycelium. Different parameters, including colony diameter, growth rate, and colony fresh weight, were evaluated, both in the presence and absence of these encapsulant agents. The results obtained confirm the ability of CDs to stimulate the growth of T. claveryi mycelium when present in the culture medium. A similar effect was observed when CDs were added to the culture medium of Tuber melanosporum. Three natural (α-, β-, and γ) and two modified (hydroxypropil-β and methyl-β) CDs were assayed. The best results were obtained with β-cyclodextrin, but no improvement was observed with its chemically modified derivatives. CDs complex the different compounds present in the culture medium which impair mycelial growth.
软珊瑚 Terfezia claveryi 具有重要的美食、营养和抗氧化特性，商业价值很高，因此人们对其栽培越来越感兴趣。然而，T. claveryi 菌丝在体外的生长不稳定且缓慢，这给获得菌根植物带来了困难，因此需要寻找一种新的培养基，以克服这些缺点。在这项工作中，我们分析了环糊精（CDs）对 T. claveryi 菌丝生长的影响。评估了不同参数，包括菌落直径、生长速度和菌落鲜重，这些参数在有和没有这些包埋剂的情况下都进行了评估。结果证实，CDs 能够在培养基中刺激 T. claveryi 菌丝的生长。当将 CDs 添加到 Tuber melanosporum 的培养基中时，也观察到了类似的效果。测试了三种天然（α-、β-和 γ-）和两种改性（羟丙基-β 和甲基-β）的 CDs。β-环糊精的效果最好，但化学改性衍生物没有改善。CDs 可以与培养基中抑制菌丝生长的不同化合物形成复合物。
