Department of Pest Control of Stored Products and Food Safety, Laboratory of Proteomics, Crop Research Institute, Drnovska507/73, Praha 6 Ruzyne, CZ-161 06, Czechia.
J Med Entomol. 2013 Jul;50(4):931-3. doi: 10.1603/me13027.
House dust mites produce antibacterial proteins suppressing bacterial growth. The 14.5-kDa bacteriolytic protein (UniProtKB Q8MWR6) has been known in Dermatophagoides pteronyssinus Trouessart. We have applied polymerase chain reaction and reverse transcription-PCR to detect a homologous gene sequence coding for a Q8MWR6-related protein in Dermatophagoides farinae (Hughes) using genomic DNA and total RNA, respectively. The resulting PCR product of expected size, 243 bp, was obtained from both Dermatophagoides spp., while no amplification was achieved from stored product mite samples. Sequence of the gene fragment from D. farinae showed 83% similarity to the previously described one in D. pteronyssinus. Successful amplification of the expected product from cDNA generated with oligo-dT primer implies that the NlpC/P60-like protein in Dermatophagoides mites is of eukaryotic or mite origin.
屋尘螨产生具有抑制细菌生长作用的抗菌蛋白。14.5kDa 溶菌素蛋白（UniProtKB Q8MWR6）已在特应性粉尘螨中被发现。我们应用聚合酶链反应和反转录-PCR 分别使用基因组 DNA 和总 RNA 从粉尘螨中检测到编码 Q8MWR6 相关蛋白的同源基因序列。从两种粉尘螨中均获得了预期大小（243bp）的 PCR 产物，而从储存产品螨样本中则未获得扩增。从 D. farinae 基因片段的序列显示与之前在 D. pteronyssinus 中描述的序列有 83%的相似性。使用 oligo-dT 引物从 cDNA 生成预期产物的成功扩增表明，粉尘螨中的 NlpC/P60 样蛋白是真核生物或螨的起源。
