Department of Medical Parasitology, Wannan Medical College, Wuhu, 241002, Anhui, China.
Department of Laboratory Medicine, Third Affiliated Hospital of Naval Medical University, Shanghai, 200438, China.
Sci Rep. 2024 Jul 12;14(1):16156. doi: 10.1038/s41598-024-66043-8.
Dermatophagoides farina (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus) are the prevalent kinds of house dust mites (HDMs). HDMs are common inhalant allergens that cause a range of allergic diseases, such as rhinitis, atopic dermatitis, and asthma. The epidemiology of these diseases is associated with exposure to mites. Therefore, in the present study, a method named multiplex loop-mediated isothermal amplification (LAMP) was developed to detect environmental dust mites. The multiplex LAMP assay allows amplification within a single tube and has an ITS plasmid detection limit as low as 40 fg/µL for both single dust mites and mixed dust mites (D. pteronyssinus and D. farinae), which is up to ten times more sensitive than classical PCR techniques. Furthermore, the multiplex LAMP method was applied to samples of single dust mites and clinical dust to confirm its validity. The multiplex LAMP assay exhibited higher sensitivity, simpler instrumentation, and visualization of test results, indicating that this method could be used as an alternative to traditional techniques for the detection of HDMs.
粉尘螨（Dermatophagoides farina，D. farinae）和屋尘螨（Dermatophagoides pteronyssinus，D. pteronyssinus）是常见的屋尘螨。屋尘螨是常见的吸入性过敏原，可引起多种过敏性疾病，如鼻炎、特应性皮炎和哮喘。这些疾病的流行病学与接触螨虫有关。因此，本研究开发了一种称为多重环介导等温扩增（LAMP）的方法来检测环境尘螨。多重 LAMP 检测可以在单个管内进行扩增，对于单个尘螨和混合尘螨（屋尘螨和粉尘螨），其 ITS 质粒检测限低至 40 fg/µL，比经典 PCR 技术灵敏 10 倍以上。此外，该多重 LAMP 方法还应用于单个尘螨和临床尘螨样本中，以确认其有效性。该多重 LAMP 检测法具有更高的灵敏度、更简单的仪器以及检测结果的可视化，表明该方法可替代传统技术用于检测 HDM。
