氢/氘交换质谱法探测蛋白质治疗药物的高级结构：方法学与应用。
Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications.
机构信息
Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Princeton, NJ, USA.
Biologics Manufacturing and Process Development, Global Manufacturing and Supply, Bristol-Myers Squibb, Hopewell, NJ, USA.
出版信息
Drug Discov Today. 2014 Jan;19(1):95-102. doi: 10.1016/j.drudis.2013.07.019. Epub 2013 Aug 6.
Abstract
The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.
摘要
蛋白质治疗药物的高级结构可以用氢/氘交换质谱（HDX-MS）来检测。HDX-MS 现在是蛋白质治疗药物结构特征描述的一种广泛应用的工具。在这篇综述中，我们展示了基于 HDX-MS 的工作流程在蛋白质治疗药物的发现和开发过程中的具体应用，重点是蛋白质/药物相互作用和生物仿制药可比性研究中的表位作图的特定应用。还描述了 HDX-MS 在蛋白质治疗药物特征描述中的应用的未来趋势。
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