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Defining a protective epitope on factor H binding protein, a key meningococcal virulence factor and vaccine antigen.定义因子 H 结合蛋白上的保护性表位，因子 H 结合蛋白是脑膜炎球菌关键毒力因子和疫苗抗原。
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3304-9. doi: 10.1073/pnas.1222845110. Epub 2013 Feb 8.
2
Fluoroketone inhibition of Ca(2+)-independent phospholipase A2 through binding pocket association defined by hydrogen/deuterium exchange and molecular dynamics.氟酮通过氢/氘交换和分子动力学定义的结合口袋关联抑制钙（Ca2+）非依赖性磷脂酶 A2。
J Am Chem Soc. 2013 Jan 30;135(4):1330-7. doi: 10.1021/ja306490g. Epub 2013 Jan 16.
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Which are the antibodies to watch in 2013?2013 年需要关注哪些抗体？
MAbs. 2013 Jan-Feb;5(1):1-4. doi: 10.4161/mabs.22976. Epub 2012 Dec 19.
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Characterization of epitopes recognized by monoclonal antibodies: experimental approaches supported by freely accessible bioinformatic tools.表位鉴定的单克隆抗体：实验方法支持自由获取的生物信息学工具。
Drug Discov Today. 2013 May;18(9-10):464-71. doi: 10.1016/j.drudis.2012.11.006. Epub 2012 Nov 21.
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Characterization of therapeutic antibodies and related products.治疗性抗体及相关产品的特性分析。
Anal Chem. 2013 Jan 15;85(2):715-36. doi: 10.1021/ac3032355. Epub 2012 Dec 14.
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Accessing the reproducibility and specificity of pepsin and other aspartic proteases.探究胃蛋白酶及其他天冬氨酸蛋白酶的可重复性和特异性。
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Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars.用于表征生物制药的分析工具及其对生物类似药的影响。
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The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: immunological, functional and structural characterization of the antigens.新型 B 群脑膜炎球菌多组份疫苗 4CMenB：抗原的免疫原性、功能性和结构特征。
Vaccine. 2012 May 30;30 Suppl 2(0 2):B87-97. doi: 10.1016/j.vaccine.2012.01.033.
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Fragmentation hydrogen exchange mass spectrometry: a review of methodology and applications.碎片化氢交换质谱法：方法与应用综述
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Marketed therapeutic antibodies compendium. marketed therapeutic antibodies compendium.
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氢/氘交换质谱法探测蛋白质治疗药物的高级结构：方法学与应用。

Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications.

机构信息

Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Princeton, NJ, USA.

Biologics Manufacturing and Process Development, Global Manufacturing and Supply, Bristol-Myers Squibb, Hopewell, NJ, USA.

出版信息

Drug Discov Today. 2014 Jan;19(1):95-102. doi: 10.1016/j.drudis.2013.07.019. Epub 2013 Aug 6.

DOI:10.1016/j.drudis.2013.07.019
PMID:23928097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4081482/
Abstract

The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.

摘要

蛋白质治疗药物的高级结构可以用氢/氘交换质谱（HDX-MS）来检测。HDX-MS 现在是蛋白质治疗药物结构特征描述的一种广泛应用的工具。在这篇综述中，我们展示了基于 HDX-MS 的工作流程在蛋白质治疗药物的发现和开发过程中的具体应用，重点是蛋白质/药物相互作用和生物仿制药可比性研究中的表位作图的特定应用。还描述了 HDX-MS 在蛋白质治疗药物特征描述中的应用的未来趋势。