细胞衰老培养方法。
Cell senescence culturing methods.
作者信息
Chen Huaping, Li Yuanyuan, Tollefsbol Trygve O
机构信息
Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
出版信息
Methods Mol Biol. 2013;1048:1-10. doi: 10.1007/978-1-62703-556-9_1.
Abstract
Development of therapeutic approaches that slow or ablate the adverse physiological and pathological changes associated with aging has been considered as an important goal for gerontological research. As cellular senescence is characterized as the basis for aging in organisms, culturing and subculturing of normal human diploid fibroblasts to mimic the in vivo aging processes have been developed as major methods to investigate molecular events involved in aging. It has been established that normal human diploid fibroblasts can proliferate in culture for only finite periods of time. There are many ways to study aging in vitro. In this chapter, we will discuss some of the basic laboratory procedures for cell senescence culturing methods.
摘要
开发能够减缓或消除与衰老相关的不良生理和病理变化的治疗方法，一直被视为老年学研究的一个重要目标。由于细胞衰老被认为是生物体衰老的基础，因此培养和传代培养正常人二倍体成纤维细胞以模拟体内衰老过程，已成为研究衰老相关分子事件的主要方法。已经确定，正常人二倍体成纤维细胞在培养中只能增殖有限的时间。体外研究衰老有很多方法。在本章中，我们将讨论细胞衰老培养方法的一些基本实验室程序。
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