衰老细胞中热量限制生物标志物的分析。
Analysis of biomarkers of caloric restriction in aging cells.
作者信息
Li Yuanyuan, Tollefsbol Trygve O
机构信息
Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
出版信息
Methods Mol Biol. 2013;1048:19-29. doi: 10.1007/978-1-62703-556-9_3.
Caloric restriction (CR) has been extensively documented for its profound role in effectively extending maximum lifespan in many different species. However, the accurate mechanisms, especially at the cellular level, for CR-induced aging delay are still under intense investigation. An emerging technique, recently explored in our laboratory, provides precisely controllable caloric intake in a cultured cellular system that allows real-time observation and quantitative analysis of the impact of CR on the molecular cellular level during the aging processes. This in vitro method allows investigation of the molecular mechanisms pertaining to how CR influences aging processes leading to life extension in human cellular systems. It will provide important clinical implications for future preventive approaches for aging and aging-related degeneration diseases in humans. Hence, we will discuss the detailed procedures of this novel technique as well as the analysis of relevant aging biomarkers and its broad application in the field.
热量限制（CR）在有效延长许多不同物种的最大寿命方面所起的重要作用已被广泛记录。然而，CR诱导衰老延迟的精确机制，尤其是在细胞水平上的机制，仍在深入研究中。我们实验室最近探索的一种新兴技术，在培养的细胞系统中提供了精确可控的热量摄入，这使得在衰老过程中能够实时观察和定量分析CR在分子细胞水平上的影响。这种体外方法可以研究与CR如何影响人类细胞系统中导致寿命延长的衰老过程相关的分子机制。它将为未来人类衰老及衰老相关退行性疾病的预防方法提供重要的临床启示。因此，我们将讨论这项新技术的详细程序以及相关衰老生物标志物的分析及其在该领域的广泛应用。
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