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大鼠毛囊干细胞及其分泌组的分离、扩增与鉴定：对伤口愈合潜力的见解

Isolation, Expansion, and Characterization of Rat Hair Follicle Stem Cells and Their Secretome: Insights into Wound Healing Potential.

作者信息

Sousa Patrícia, Lopes Bruna, Sousa Ana Catarina, Coelho André, de Sousa Moreira Alícia, Rêma Alexandra, Gonçalves-Maia Maria, Amorim Irina, Alvites Rui, Alves Nuno, Geuna Stefano, Maurício Ana Colette

机构信息

Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal.

Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.

出版信息

Biomedicines. 2024 Dec 15;12(12):2854. doi: 10.3390/biomedicines12122854.

DOI:10.3390/biomedicines12122854
PMID:39767760
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11672956/
Abstract

Stem cells are capable of self-renewal and differentiation into various specialized cells, making them a potential therapeutic option in regenerative medicine. This study establishes a comprehensive methodology for isolating, culturing, and characterizing rat hair follicle stem cells. Hair follicles were harvested from Sprague-Dawley rats and subjected to two different isolation techniques. Immunohistochemical analysis and real-time PCR confirm the expression of specific surface markers and genes, validating the cells' identity. Growth kinetics, colony formation units (CFU), and tri-differentiation capacity were also assessed. Additionally, the cells' secretome was analyzed, regarding its content in biofactors with wound healing properties. These findings highlight the potential of these cells as a valuable cell source for skin regeneration applications. They contribute to advancing our understanding of stem cell applications in regenerative medicine and hold promise for therapeutic interventions in various clinical contexts, aligning with broader research on the diverse capabilities of hair follicle stem cells.

摘要

干细胞能够自我更新并分化为各种特化细胞，这使其成为再生医学中一种潜在的治疗选择。本研究建立了一套全面的方法，用于分离、培养和鉴定大鼠毛囊干细胞。从斯普拉格-道利大鼠身上采集毛囊，并采用两种不同的分离技术。免疫组织化学分析和实时聚合酶链反应证实了特定表面标志物和基因的表达，验证了细胞的身份。还评估了生长动力学、集落形成单位（CFU）和三向分化能力。此外，分析了细胞的分泌组，研究其在具有伤口愈合特性的生物因子中的含量。这些发现突出了这些细胞作为皮肤再生应用中有价值的细胞来源的潜力。它们有助于推进我们对干细胞在再生医学中应用的理解，并有望在各种临床情况下进行治疗干预，与关于毛囊干细胞多种能力的更广泛研究相一致。

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