Human Influenza Department, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune, 411001, India,
Arch Virol. 2014 Feb;159(2):217-25. doi: 10.1007/s00705-013-1812-6. Epub 2013 Aug 9.
Human metapneumovirus (HMPV) is an important respiratory virus implicated in respiratory infections. The purpose of this study was to develop a one-step real-time RT-PCR assay that can detect all four lineages of HMPV and to identify the HMPV lineages circulating in Pune, India. Conserved regions of the nucleoprotein gene were used to design real-time primers and a probe. A total of 224 clinical samples that were positive for different respiratory viruses (including 51 samples that were positive for HMPV) were tested using the real time RT-PCR assay, and the specificity of the assay was observed to be 100 %. Using in vitro-synthesized RNA, the sensitivity of the assay was ascertained to be 100 copies of the target gene per reaction. Phylogenetic analysis of the nucleoprotein (N) and attachment glycoprotein (G) genes confirmed that this assay detected all lineages of HMPV. A2, B1 and B2 strains were observed during the study period. Our assay is highly sensitive and specific for all known lineages of HMPV, making it a valuable tool for rapid detection of the virus. A2 and B2 were the predominant subtypes circulating in Pune, Western India.
人偏肺病毒（HMPV）是一种重要的呼吸道病毒，与呼吸道感染有关。本研究旨在开发一种可检测所有四种 HMPV 谱系的一步实时 RT-PCR 检测方法，并鉴定印度浦那流行的 HMPV 谱系。使用核蛋白基因的保守区设计实时引物和探针。使用实时 RT-PCR 检测方法检测了 224 份检测出不同呼吸道病毒（包括 51 份 HMPV 阳性样本）的临床样本，该检测方法的特异性为 100%。使用体外合成的 RNA 确定了该检测方法的敏感性为每个反应 100 个目标基因拷贝。核蛋白（N）和附着糖蛋白（G）基因的系统进化分析证实，该检测方法可检测到所有 HMPV 谱系。在研究期间观察到 A2、B1 和 B2 株。我们的检测方法对所有已知的 HMPV 谱系高度敏感和特异，是快速检测该病毒的有价值的工具。A2 和 B2 是在印度西部浦那流行的主要亚型。
