Agroprocessing and Natural Products Division, Council of Scientific and Industrial Research, National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram-, 695019, Kerala, India.
J Sci Food Agric. 2014 Mar 30;94(5):943-50. doi: 10.1002/jsfa.6340. Epub 2013 Sep 4.
Rapid urbanisation and nutritional transition is fuelling the increased global incidence of type 2 diabetes. Pineapple fruit residue was explored for its nutraceutical properties as an alternative or adjunct to currently available treatment regime. Ethyl acetate and methanolic extracts of pineapple fruit residue were evaluated for anti-diabetic activity in cell free and cell based systems. Specifically, we assessed: (1) antioxidant potential, (2) anti-glycation potential, (3) carbohydrate digestive enzyme inhibition, and (4) lipid accumulation and glycerol-3-phosphate dehydrogenase activity in differentiating 3T3-L1 cells.
The active components in the ethyl acetate and methanolic extracts were identified as sinapic acid, daucosterol, 2-methylpropanoate, 2,5-dimethyl-4-hydroxy-3(2H)-furanone, methyl 2-methylbutanoate and triterpenoid ergosterol using DART/HRMS and ESI/HRMS. Micronutrient analysis revealed the presence of magnesium, potassium and calcium. Adipogenic potential, anti-glycation property of the ethyl acetate extract, and DNA damage protection capacity of the methanolic extract are promising.
Results from this study clearly indicate that pineapple fruit residue could be utilised as a nutraceutical against diabetes and related complications.
快速的城市化和营养转型正在推动全球 2 型糖尿病发病率的上升。菠萝果渣因其具有作为现有治疗方案的替代或辅助的营养特性而受到探索。评估了菠萝果渣的乙酸乙酯和甲醇提取物在无细胞和基于细胞的系统中的抗糖尿病活性。具体来说，我们评估了：（1）抗氧化潜力，（2）抗糖化潜力，（3）碳水化合物消化酶抑制作用，以及（4）分化的 3T3-L1 细胞中的脂质积累和甘油-3-磷酸脱氢酶活性。
使用 DART/HRMS 和 ESI/HRMS 鉴定出乙酸乙酯和甲醇提取物中的活性成分是芥子酸、豆甾醇、2-甲基丙酯、2,5-二甲基-4-羟基-3(2H)-呋喃酮、甲基 2-甲基丁酸酯和三萜类麦角固醇。微量营养素分析显示存在镁、钾和钙。成脂潜力、乙酸乙酯提取物的抗糖化特性以及甲醇提取物的 DNA 损伤保护能力都很有前景。
本研究结果清楚地表明，菠萝果渣可用作防治糖尿病及相关并发症的营养保健品。
