Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Gyeonggi-do, Republic of Korea.
Department of Food Science and Biotechnology, Dongguk University-Seoul, Gyeonggi-do, Korea.
PLoS One. 2019 Aug 12;14(8):e0220950. doi: 10.1371/journal.pone.0220950. eCollection 2019.
Currently, green nanotechnology-based approaches using waste materials from food have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the outer peel extract of the fruit Ananas comosus (AC), which is a food waste material. Characterization was done using UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-visible spectroscopy (at 485 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in AC outer peel extract accountable for the reduction of Ag+ ion and the stabilization of AC-AgNPs was investigated through FT-IR. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AC-AgNPs were evaluated for their antioxidative, antidiabetic, and cytotoxic potential against HepG2 cells along with their antibacterial potential. The results showed that AC-AgNPs are extremely effective with high antidiabetic potential at a very low concentration as well as it exhibited higher cytotoxic activity against the HepG2 cancer cells in a dose-dependent manner. It also exhibited potential antioxidant activity and moderate antibacterial activity against the four tested foodborne pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AC-AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. Further, it has applications in wound dressing or in treating bacterial related diseases.
目前，利用食品废弃物的绿色基于纳米技术的方法已被认为是一种具有环境友好和成本效益的方法，具有多种生物医学应用。在本研究中，使用水果菠萝（AC）的外皮提取物合成 AgNPs，这是一种食品废弃物。通过紫外可见光谱、X 射线衍射（XRD）、傅里叶变换红外（FT-IR）光谱、扫描电子显微镜（SEM）和能谱分析（EDX）进行了表征。AgNPs 的形成通过紫外可见光谱（在 485nm 处）得到了确认，这是由于表面等离子体共振引起的颜色变化。根据 XRD 图谱，确定了 AgNPs 的结晶性质。通过 FT-IR 研究了 AC 外皮提取物中存在的功能基团，该基团负责还原 Ag+离子并稳定 AC-AgNPs。通过 SEM 和 EDX 分析确定了形态结构和元素组成。随着 AgNPs 在生物医学方面的应用不断增加，对生物合成的 AC-AgNPs 进行了抗氧化、抗糖尿病和对 HepG2 细胞的细胞毒性以及抗菌潜力的评估。结果表明，AC-AgNPs 非常有效，在非常低的浓度下具有很高的抗糖尿病潜力，并且对 HepG2 癌细胞具有更高的细胞毒性活性，呈剂量依赖性。它还表现出潜在的抗氧化活性和对四种测试的食源性病原体的适度抗菌活性。总的来说，这些结果突出了 AC-AgNPs 在生物医学领域的有效性和潜在应用，例如在急性疾病的治疗以及治疗癌症和糖尿病等各种疾病的药物配方中。此外，它还可应用于伤口敷料或治疗细菌相关疾病。
