Altun Gokalp, Cakiroglu Yavuz, Pulathan Zerrin, Yulug Esin, Mentese Ahmet
Department of Cardiovascular Surgery, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
Iran J Basic Med Sci. 2020 Feb;23(2):271-276. doi: 10.22038/IJBMS.2019.36215.8626.
The aim of this study is to investigate the renoprotective effect of erythropoietin (EPO) on hypovolemic shock and ischemia/reperfusion (IR) injury on kidneys as end-organs in an experimentally-created ruptured abdominal aortic aneurysm (rAAA) model.
Thirty anesthetized Sprague-Dawley male rats were randomized to sham ((Sh n:6) (Sh+EPO n:6)) or shock and I/R groups ((S/IR n:9) (S/IR+EPO n:9)). Additional surgical procedure except aortic exploration was not performed on Sh and Sh+EPO groups. 60 min of shock, 60 min of ischemia, and 120 min of reperfusion were applied on S/IR and S/IR+EPO groups. In the S/IR and S/IR+EPO groups, hemorrhagic shock, lower torso ischemia, and reperfusion were created. At the end of the shock period, saline solutions were separately and equally administered to Sh and S/IR groups, whereas 2000 U/kg EPO was intraperitoneally administered to Sh+EPO and S/IR+EPO groups. At the end of the experimental study, some biochemical and histological parameters were studied in serum and kidney tissues.
Biochemical parameters were all significantly increased in the S/IR group compared with the Sh group. These parameters were not statistically significantly different between S/IR+EPO and Sh+EPO groups. In histopathologic examination, EPO prevented high-grade injury.
Our data indicate that EPO may have a renoprotective effect and reduce the systemic inflammatory response that resulted from shock and I/R in an experimental model of rAAA.
本研究旨在探讨在实验性创建的腹主动脉瘤破裂（rAAA）模型中，促红细胞生成素（EPO）对低血容量性休克以及作为终末器官的肾脏缺血/再灌注（IR）损伤的肾脏保护作用。
将30只麻醉的雄性Sprague-Dawley大鼠随机分为假手术组（（Sh n:6）（Sh+EPO n:6））或休克与缺血/再灌注组（（S/IR n:9）（S/IR+EPO n:9））。Sh组和Sh+EPO组除主动脉探查外未进行其他手术操作。S/IR组和S/IR+EPO组施加60分钟休克、60分钟缺血和120分钟再灌注。在S/IR组和S/IR+EPO组中，造成失血性休克、下半身缺血和再灌注。休克期结束时，Sh组和S/IR组分别等量给予生理盐水，而Sh+EPO组和S/IR+EPO组腹腔注射2000 U/kg EPO。实验研究结束时，对血清和肾脏组织中的一些生化和组织学参数进行了研究。
与Sh组相比，S/IR组的生化参数均显著升高。S/IR+EPO组和Sh+EPO组之间这些参数无统计学显著差异。在组织病理学检查中，EPO预防了高级别损伤。
我们的数据表明，在rAAA实验模型中，EPO可能具有肾脏保护作用，并减少休克和缺血/再灌注引起的全身炎症反应。
