Allain P, Mauras Y, Dougé C, Jaunault L, Delaporte T, Beaugrand C
Laboratoire de Pharmacologie, Centre Hospitalier Universitaire, France.
Analyst. 1990 Jun;115(6):813-5. doi: 10.1039/an9901500813.
The simultaneous determination of iodine and bromine in plasma and urine by inductively coupled plasma mass spectrometry, using a Nermag prototype instrument, is described. The sample preparation involves only a 10-fold dilution with a diluent containing europium as an internal standard followed by direct nebulisation in the plasma. The iodine, bromine and europium ions are measured at m/z = 127, 79, and 153, respectively. The sensitivity of the method, with detection limits of 1.6 and 52 micrograms l-1 for iodine and bromine, respectively, is satisfactory for clinical applications. The calibration graphs were linear over the ranges 0-400 micrograms l-1 and 0-40 mg l-1 for iodine and bromine, respectively, which are wide enough for most assays. The recoveries were close to 100% with coefficients of variation of less than 3%. The within-day and between-day reproducibility was about 5%. The concentrations of iodine and bromine in the plasma of 26 healthy individuals were 58 +/- 12 micrograms l-1 and 4.1 +/- 0.9 mg l-1, respectively. The amounts of iodine and bromine eliminated in urine were 94 +/- 97 micrograms per 24 h (range 27-403 micrograms per 24 h) and 3.6 +/- 1.7 mg per 24 h, respectively. These results are in agreement with reported values.
本文介绍了使用Nermag原型仪器通过电感耦合等离子体质谱法同时测定血浆和尿液中的碘和溴。样品制备仅需用含铕作为内标的稀释剂进行10倍稀释，然后直接在等离子体中雾化。碘、溴和铕离子分别在m/z = 127、79和153处测量。该方法的灵敏度分别为碘1.6微克/升和溴52微克/升，对临床应用来说是令人满意的。校准曲线在碘的0 - 400微克/升和溴的0 - 40毫克/升范围内呈线性，这对大多数分析来说足够宽。回收率接近100%，变异系数小于3%。日内和日间重现性约为5%。26名健康个体血浆中碘和溴的浓度分别为58±12微克/升和4.1±0.9毫克/升。尿中排出的碘和溴量分别为每24小时94±97微克（范围为每24小时27 - 403微克）和每24小时3.6±1.7毫克。这些结果与报道值一致。
