使用双探头单光子发射计算机断层扫描/计算机断层扫描（DISA-SPECT/CT）和3.0T心脏磁共振成像（MRI）检测和定量兔急性再灌注心肌梗死
Detection and quantification of acute reperfused myocardial infarction in rabbits using DISA-SPECT/CT and 3.0T cardiac MRI.
作者信息
Feng Yuanbo, Cona Marlein Miranda, Vunckx Kathleen, Li Yue, Chen Feng, Nuyts Johan, Gheysens Olivier, Zhou Lin, Xie Yi, Oyen Raymond, Ni Yicheng
机构信息
Theragnostic Laboratory and Radiology Section, Department of Imaging and Pathology, Biomedical Sciences Group, KU Leuven, Leuven, Belgium.
出版信息
Int J Cardiol. 2013 Oct 9;168(4):4191-8. doi: 10.1016/j.ijcard.2013.07.108. Epub 2013 Aug 6.
BACKGROUND
Necrosis avid tracer (123)I-hypericin ((123)I-HYP) enables hot-spot imaging on acute myocardial infarction (MI). We explored dual-isotope simultaneous acquisition single photon emission computed tomography/computed tomography (DISA-SPECT/CT) by using (123)I-HYP and standard (99m)Tc-sestamibi ((99m)Tc-MIBI), in comparison with cardiac magnetic resonance imaging (cMRI), autoradiography (AutoRx) and histomorphometry.
METHODS
Acute MI was induced by 90-min coronary artery occlusion and 24-h reperfusion in 9 rabbits. They were scanned with cMRI at 3.0T, followed by intravenous injections of (123)I-HYP, and 8h later, of (99m)Tc-MIBI. Then, they were imaged with DISA-SPECT/CT for detection and localization of MI. The excised heart was sectioned for AutoRx, triphenyltetrazolium chloride (TTC) histochemistry, and haematoxylin-eosin (HE) staining. DISA-SPECT/CT and cMRI were co-registered, and MI was compared between different modalities and techniques for correlation with ex vivo findings. Tracer/contrast uptakes were quantified on polar maps. One way-ANOVA and Bonferroni's tests were used for comparison of multiple techniques. Linear regression and Bland-Altman analysis were used to compare measurements of MI.
RESULTS
MI volumes were not significantly different as by (99m)Tc-MIBI-SPECT, (123)I-HYP-SPECT, cMRI and TTC (38.94 ± 13.97%, 37.76 ± 13.16%, 35.19 ± 12.53% and 33.26 ± 10.65%; p > 0.05). The MI areas were 41.13 ± 18.70%, 40.19 ± 18.45%, 38.23 ± 16.86%, 36.83 ± 16.70%, 36.16 ± 16.15% and 35.03 ± 14.75% on (99m)Tc-MIBI-SPECT, (123)I-HYP-SPECT, cMRI, AutoRx, TTC and HE. There was no significant differences between each of two techniques (p = 0.9). Tracer/contrast uptakes were well correlated ((123)I-HYP vs (99m)Tc-MIBI r(2) = 0.66; (123)I-HYP vs cMRI r(2) = 0.63; (99m)Tc-MIBI vs cMRI r(2) = 0.64). Infarct/normal myocardium activity ratio was 40/1 and 23/1 by AutoRx and γ-counting.
CONCLUSION
(123)I-HYP has shown pronounced necrosis-avidity, which proves complementary for imaging MI with potential clinical applicability for myocardial viability determination.
背景
坏死亲和性示踪剂（123）I-金丝桃素（（123）I-HYP）可实现急性心肌梗死（MI）的热点成像。我们探索了使用（123）I-HYP和标准（99m）Tc-甲氧基异丁基异腈（（99m）Tc-MIBI）的双同位素同时采集单光子发射计算机断层扫描/计算机断层扫描（DISA-SPECT/CT），并与心脏磁共振成像（cMRI）、放射自显影（AutoRx）和组织形态计量学进行比较。
方法
对9只兔子进行90分钟冠状动脉闭塞和24小时再灌注诱导急性心肌梗死。在3.0T条件下用cMRI对它们进行扫描，随后静脉注射（123）I-HYP，8小时后注射（99m）Tc-MIBI。然后，用DISA-SPECT/CT对它们进行成像以检测和定位心肌梗死。将切除的心脏切片进行放射自显影、氯化三苯基四氮唑（TTC）组织化学和苏木精-伊红（HE）染色。对DISA-SPECT/CT和cMRI进行配准，并比较不同模式和技术之间的心肌梗死情况，以与离体结果进行相关性分析。在极坐标图上对示踪剂/造影剂摄取进行定量。采用单因素方差分析和Bonferroni检验比较多种技术。使用线性回归和Bland-Altman分析比较心肌梗死的测量值。
结果
通过（99m）Tc-MIBI-SPECT、（123）I-HYP-SPECT、cMRI和TTC测得的心肌梗死体积无显著差异（分别为38.94±13.97%、37.76±13.16%、35.19±12.53%和33.26±10.65%；p>0.05）。在（99m）Tc-MIBI-SPECT、（123）I-HYP-SPECT、cMRI、放射自显影、TTC和HE上测得的心肌梗死面积分别为41.13±18.70%、40.19±18.45%、38.23±16.86%、36.83±16.70%、36.16±16.15%和35.03±14.75%。两种技术之间均无显著差异（p = 0.9）。示踪剂/造影剂摄取具有良好的相关性（（123）I-HYP与（99m）Tc-MIBI的r（2）= 0.66；（123）I-HYP与cMRI的r（2）= 0.
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