Klemens S P, Cynamon M H, Swenson C E, Ginsberg R S
Veterans Administration Medical Center, Syracuse, New York.
Antimicrob Agents Chemother. 1990 Jun;34(6):967-70. doi: 10.1128/AAC.34.6.967.
The efficacy of liposome-encapsulated gentamicin and free gentamicin was evaluated with the beige (C57BL/6J-bgj/bgj) mouse model of disseminated Mycobacterium avium complex infection. Approximately 10(7) viable M. avium complex cells were given intravenously. Seven days later, treatment with either encapsulated or free gentamicin at 20 mg/kg of body weight was started. Treatment was given daily for 5 consecutive days or twice weekly for 3 weeks. The mice were sacrificed 5 days after the last dose. Spleens, livers, and lungs were homogenized, and viable cell counts were determined. An analysis of variance and subsequent Tukey honestly significant difference tests indicated that both encapsulated and free gentamicin reduced viable cell counts in each of the organs compared with no treatment. Encapsulated gentamicin significantly reduced viable cell counts in the spleen and liver compared with the free gentamicin. A dose-response experiment was performed with a daily dose of 0.2, 2, or 20 mg/kg. Dose-related reductions in viable cell counts were observed for spleens and livers, although none of the regimens resulted in sterilization of these organs. Liposome-encapsulated gentamicin should be considered for further evaluation in the treatment of M. avium complex infection in humans.
采用米色（C57BL/6J-bgj/bgj）小鼠播散性鸟分枝杆菌复合群感染模型，评估脂质体包裹庆大霉素和游离庆大霉素的疗效。静脉注射约10(7)个活的鸟分枝杆菌复合群细胞。7天后，开始以20mg/kg体重的剂量用包裹或游离庆大霉素进行治疗。连续5天每日给药，或每周两次给药3周。最后一剂给药5天后处死小鼠。将脾脏、肝脏和肺组织匀浆，测定活菌数。方差分析及随后的Tukey真实显著差异检验表明，与未治疗相比，包裹和游离庆大霉素均降低了各器官中的活菌数。与游离庆大霉素相比，包裹庆大霉素显著降低了脾脏和肝脏中的活菌数。进行了每日剂量为0.2、2或20mg/kg的剂量反应实验。观察到脾脏和肝脏中活菌数与剂量相关的减少，尽管没有一种方案能使这些器官灭菌。脂质体包裹庆大霉素应考虑在人类鸟分枝杆菌复合群感染治疗中进行进一步评估。
