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一种改进的牡蛎中创伤弧菌定量方法。

An improved method for quantification of Vibrio vulnificus in oysters.

机构信息

The New Zealand Institute for Plant & Food Research, Limited Private Bag 92169, Auckland 1142, New Zealand.

出版信息

J Microbiol Methods. 2013 Dec;95(3):397-9. doi: 10.1016/j.mimet.2013.07.024. Epub 2013 Aug 7.

DOI:10.1016/j.mimet.2013.07.024
PMID:23933605
Abstract

Chromogenic agar was compared with the FDA recommended cellobiose-colistin agar for assessment of Vibrio vulnificus in oysters. A two-step culture confirmation method was also evaluated. The inclusion of CA gave a 33% increase in the detection rate and the two-step culture confirmation eliminated 62.5% of false positives.

摘要

显色琼脂与美国食品药品监督管理局推荐的纤维二糖-黏菌素琼脂用于牡蛎中创伤弧菌的评估进行了比较。还评估了两步培养确认方法。添加 CA 可将检测率提高 33%,两步培养确认可消除 62.5%的假阳性。

相似文献

1
An improved method for quantification of Vibrio vulnificus in oysters.一种改进的牡蛎中创伤弧菌定量方法。
J Microbiol Methods. 2013 Dec;95(3):397-9. doi: 10.1016/j.mimet.2013.07.024. Epub 2013 Aug 7.
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The evaluation of four recent culture-based methods for the isolation and enumeration of Vibrio vulnificus bacteria from oyster meat.四种基于培养的最近方法对牡蛎肉中创伤弧菌的分离和计数的评估。
J Microbiol Methods. 2014 Feb;97:1-5. doi: 10.1016/j.mimet.2013.12.004. Epub 2013 Dec 16.
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Methods for isolation and confirmation of Vibrio vulnificus from oysters and environmental sources: a review.从牡蛎和环境源中分离与鉴定创伤弧菌的方法:综述
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Reduction of Vibrio vulnificus in pure culture, half shell and whole shell oysters (Crassostrea virginica) by X-ray.通过X射线减少纯培养物、半壳和全壳牡蛎(弗吉尼亚巨蛎)中的创伤弧菌。
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Detection and enumeration of Vibrio vulnificus in oysters from two estuaries along the southwest coast of India, using molecular methods.采用分子方法对印度西南海岸两个河口的牡蛎中创伤弧菌进行检测和计数。
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Real-time PCR detection of Vibrio vulnificus in oysters: comparison of oligonucleotide primers and probes targeting vvhA.牡蛎中创伤弧菌的实时聚合酶链反应检测:针对vvhA的寡核苷酸引物和探针的比较
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A chromogenic substrate culture plate for early identification of Vibrio vulnificus and isolation of other marine Vibrios.一种用于早期鉴定创伤弧菌及分离其他海洋弧菌的显色底物培养平板。
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Inactivation of Vibrio parahaemolyticus and Vibrio vulnificus in oysters by high-hydrostatic pressure and mild heat.高压和温和热处理对牡蛎中副溶血性弧菌和创伤弧菌的灭活作用。
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Evaluation of postharvest-processed oysters by using PCR-based most-probable-number enumeration of Vibrio vulnificus bacteria.使用基于聚合酶链反应的创伤弧菌最可能数计数法对收获后处理的牡蛎进行评估。
Appl Environ Microbiol. 2007 Nov;73(22):7477-81. doi: 10.1128/AEM.01118-07. Epub 2007 Sep 28.

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