UNC-Chapel Hill, Institute of Marine Sciences, 3431 Arendell Street, Morehead City, NC 28557, USA.
UNC-Chapel Hill, Institute of Marine Sciences, 3431 Arendell Street, Morehead City, NC 28557, USA.
J Microbiol Methods. 2014 Feb;97:1-5. doi: 10.1016/j.mimet.2013.12.004. Epub 2013 Dec 16.
The most common cause of seafood-borne death in the United States is the bacterium Vibrio vulnificus which can be concentrated into high numbers in the tissues of oysters or other shellfish. The ability to quickly, accurately, and inexpensively isolate living strains of this organism from oyster tissues is crucial for effective research on this pathogen. In this report, we evaluate four methods for isolating and quantifying V. vulnificus from oyster tissues, the solid media CPC+ (a refined version of cellobiose-polymyxin B-colistin medium), CHROMagar Vibrio, VVX (Vibrio vulnificus X-gal), and a method termed "Triple plating". Up to 1225 presumptive isolates were detected by each method, and 335 were subjected to molecular typing. The selectivity and sensitivity of each method was examined and VVX was found to be the most accurate method, with each of the other methods being recommended for task-specific uses. CHROMagar Vibrio is recommended for ease of use and relative accuracy, CPC+ is best used to differentiate between clinically associated and environmental strains.
在美国，导致食源性海产品死亡的最常见原因是创伤弧菌，这种细菌在牡蛎或其他贝类的组织中可以高度集中。从牡蛎组织中快速、准确、廉价地分离出这种生物体的活菌株对于对这种病原体进行有效的研究至关重要。在本报告中，我们评估了从牡蛎组织中分离和定量创伤弧菌的四种方法，即固体培养基 CPC+（纤维二糖多粘菌素 B-多粘菌素培养基的改良版）、CHROMagar Vibrio、VVX（创伤弧菌 X-半乳糖）和一种称为“三重平板”的方法。每种方法最多可检测到 1225 个疑似分离株，并对 335 个分离株进行了分子分型。我们检查了每种方法的选择性和灵敏度，发现 VVX 是最准确的方法，其他方法则推荐用于特定任务。CHROMagar Vibrio 因其易于使用和相对准确性而被推荐，CPC+ 最适合用于区分临床相关菌株和环境菌株。
