采用分子方法对印度西南海岸两个河口的牡蛎中创伤弧菌进行检测和计数。
Detection and enumeration of Vibrio vulnificus in oysters from two estuaries along the southwest coast of India, using molecular methods.
作者信息
Parvathi Ammini, Kumar H Sanath, Karunasagar Indrani, Karunasagar Iddya
机构信息
Department of Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore, India.
出版信息
Appl Environ Microbiol. 2004 Nov;70(11):6909-13. doi: 10.1128/AEM.70.11.6909-6913.2004.
Abstract
This study was conducted to understand the seasonal distribution of Vibrio vulnificus in oysters from two estuaries and the effect of environmental factors on the abundance of V. vulnificus in tropical waters. V. vulnificus was detected in 56.6% of the samples tested by colony hybridization with an alkaline phosphatase-labeled oligonucleotide probe (VV-AP), and the counts ranged from <10/g during the summer months to 10(3)/g in the monsoon season at both sites. The density of V. vulnificus appeared to be controlled more by salinity than by temperature. A nested PCR used in this study detected V. vulnificus in 85% of the samples following 18 h of enrichment in alkaline peptone water.
摘要
本研究旨在了解两个河口牡蛎中创伤弧菌的季节分布以及环境因素对热带水域创伤弧菌丰度的影响。通过用碱性磷酸酶标记的寡核苷酸探针（VV-AP）进行菌落杂交，在56.6%的测试样本中检测到了创伤弧菌，两个地点的菌数范围从夏季的<10/g到季风季节的10³/g。创伤弧菌的密度似乎更多地受盐度而非温度的控制。本研究中使用的巢式PCR在碱性蛋白胨水中富集18小时后，在85%的样本中检测到了创伤弧菌。
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