Chang Po-Chun, Wang Chen-Ying, Chong Li Yen
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei 100, Taiwan,
Lasers Med Sci. 2015 Feb;30(2):741-6. doi: 10.1007/s10103-013-1416-0. Epub 2013 Aug 11.
Because a single exposure to light-emitting diode (LED) irradiation at 660 nm only demonstrated a 3-day biostimulatory effect in recovering periodontal bone level (PBL), this study sought to evaluate whether the periodontal effect could be extended through the use of multiple LED irradiations. Experimental periodontitis was developed unilaterally in 48 Sprague-Dawley rats after the placement of a silk ligature plus Porphyromonas gingivalis lipopolysaccharide injections. The animals were divided into four groups (no irradiation, a single irradiation, or two or three irradiations per week) and exposed to LED light irradiation at a wavelength of 660 ± 25 nm and energy density of 10 J/cm(2) after debridement and detoxification. The animals were euthanized after 7 or 14 days, and the effect of irradiation was evaluated using micro-computed tomography and histology. By day 7, PBL was significantly reduced (p < 0.05), with significantly reduced inflammation (p < 0.05) and gingival hyperplasia (p < 0.001), in the animals receiving three irradiations per week. At day 14, the reduction in gingival hyperplasia was still significant (p < 0.05), and collagen matrix deposition and realignment appeared to be accelerated in the animals receiving three irradiations per week, despite a lack of significant difference in PBL. The treatment regimen receiving three LED light irradiations per week apparently extended the effects in reducing PBL and inflammation to 7 days. The inclusion of additional inflammation control measures or the addition of bioactive signals to mediate the repairing process is necessary to maintain long-term periodontal stability.
由于单次暴露于660nm的发光二极管（LED）照射仅在牙周骨水平（PBL）恢复中显示出3天的生物刺激作用，本研究旨在评估是否可以通过多次LED照射来延长牙周效应。在48只Sprague-Dawley大鼠单侧放置丝线结扎并注射牙龈卟啉单胞菌脂多糖后诱发实验性牙周炎。将动物分为四组（不照射、单次照射或每周两次或三次照射），在清创和解毒后，暴露于波长为660±25nm、能量密度为10J/cm²的LED光照射下。7天或14天后对动物实施安乐死，并使用微型计算机断层扫描和组织学评估照射效果。到第7天，每周接受三次照射的动物的PBL显著降低（p<0.05），炎症（p<0.05）和牙龈增生（p<0.001）显著减轻。在第14天，牙龈增生的减轻仍然显著（p<0.05），尽管PBL没有显著差异，但每周接受三次照射的动物中胶原基质的沉积和重新排列似乎加速。每周接受三次LED光照射的治疗方案显然将降低PBL和炎症的效果延长至7天。有必要纳入额外的炎症控制措施或添加生物活性信号来介导修复过程，以维持长期的牙周稳定性。
