Xing Zhi-hua, Peng Wei-jun, Huang Wei, Huang Xi, Liu Wei-ping
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China.
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China TCM Pharmacogenetics Laboratory, Central South University, Changsha, China
J Chromatogr Sci. 2014 Sep;52(8):826-30. doi: 10.1093/chromsci/bmt122. Epub 2013 Aug 8.
A ultraperformance liquid chromatography (UPLC)-photodiode array (PDA) detection method was established for the simultaneous determination of seven constituents in Fructus aurantii-Magnolia bark decoction: naringin, hesperidin, neohesperidin, narirutin, meranzin hydrate, honokiol and magnolol. These were separated in <17 min using a C18 column with gradient elution using (a) acetonitrile, (b) water and (c) acetic acid at a flow rate of 0.3 mL/min, and with a PDA detector. All calibration curves showed good linear regression (r(2) > 0.9992) within the test ranges. The method was validated for specificity, accuracy, precision and limits of detection. The proposed method was found to be suitable for the quality assessment of the formula.
建立了一种超高效液相色谱（UPLC）-光电二极管阵列（PDA）检测方法，用于同时测定枳实-厚朴汤中的7种成分：柚皮苷、橙皮苷、新橙皮苷、柚皮芸香苷、水合橙皮内酯、厚朴酚和和厚朴酚。使用C18柱，以乙腈、水和乙酸为流动相进行梯度洗脱，流速为0.3 mL/min，并采用PDA检测器，在<17分钟内实现了这些成分的分离。所有校准曲线在测试范围内均显示出良好的线性回归（r(2)>0.9992）。该方法在专属性、准确性、精密度和检测限方面得到了验证。结果表明，该方法适用于该方剂的质量评价。
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