建立一种超高效液相色谱法同时测定大鼠血浆中六种活性成分及其在不同剂量给药大鼠体内的比较药代动力学研究中的应用。
Development of an Ultra-High Performance Liquid Chromatography Method for Simultaneous Determination of Six Active Compounds in and Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Rats Administered with Different Doses.
作者信息
Wang Wenbo, Zhao Linlin, Huang Huiyong, Yao Jiamei, Zhou Lu, Wang Dongsheng, Qiu Xinjian
机构信息
Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Physical Examination Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
出版信息
J Anal Methods Chem. 2018 May 10;2018:7579136. doi: 10.1155/2018/7579136. eCollection 2018.
A rapid, accurate, and sensitive ultra-high performance liquid chromatography (UHPLC) method was established for simultaneously detecting naringin, hesperidin, neohesperidin, meranzin hydrate, naringenin, and hesperetin in (FA) decoction. Analysis was performed on Waters BEH (R) C18 (50 mm × 2.1 mm, 1.7 m) at a flow rate of 0.2 mL/min by using (A) acetonitrile and (B) 0.5% acetic acid-water as the mobile phase. The method was well validated on linearity, precision, recoveries, and stability. Then, we used the same UHPLC conditions for quantitative analysis of meranzin hydrate, naringenin, and hesperetin in rat plasma. The method proved to be linear within the concentration ranges of 3.3-3300 ng/mL for meranzin hydrate, 6.95-3555 ng/mL for naringenin, and 1.8-236 ng/mL for hesperetin. The RSD of precision ranged from 1.22% to 9.08%, and the average extraction recovery ranged from 96.49 ± 1.42% to 102.01 ± 3.16%. Besides, we performed a comparative pharmacokinetic study after oral administration of FA decoction at a low dose of 15 g/kg and high dose of 30 g/kg body weight for seven days to rats. The AUC and of meranzin hydrate, naringenin, and hesperetin were multiplied significantly with the increase of FA dosage, and the of meranzin hydrate was faster than naringenin and hesperetin in the two groups.
建立了一种快速、准确、灵敏的超高效液相色谱（UHPLC）方法，用于同时检测（方剂名称未给出，暂用FA代替）方剂中的柚皮苷、橙皮苷、新橙皮苷、水合橙皮内酯、柚皮素和橙皮素。采用Waters BEH（R）C18（50 mm × 2.1 mm，1.7 μm）色谱柱，以乙腈（A）和0.5%乙酸水（B）为流动相，流速为0.2 mL/min进行分析。该方法在线性、精密度、回收率和稳定性方面均得到了良好的验证。然后，我们采用相同的UHPLC条件对大鼠血浆中的水合橙皮内酯、柚皮素和橙皮素进行定量分析。结果表明，水合橙皮内酯在3.3 - 3300 ng/mL浓度范围内、柚皮素在6.95 - 3555 ng/mL浓度范围内、橙皮素在1.8 - 236 ng/mL浓度范围内线性关系良好。精密度的相对标准偏差（RSD）为1.22%至9.08%，平均提取回收率为96.49 ± 1.42%至102.01 ± 3.16%。此外，我们对大鼠连续7天口服低剂量15 g/kg和高剂量30 g/kg体重的FA方剂进行了比较药代动力学研究。随着FA剂量的增加，水合橙皮内酯、柚皮素和橙皮素的AUC和（此处原文缺失部分内容）显著增加，且在两组中，水合橙皮内酯的（此处原文缺失部分内容）比柚皮素和橙皮素更快。
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