University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 28/30, 49149 Münster, Germany.
Metallomics. 2013 Oct;5(10):1440-7. doi: 10.1039/c3mt00147d.
A novel quantification approach for tissue imaging using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) based on tissue embedding in cold-curing resins (Technovit 7100) is presented. With respect to massive side effects on cisplatin, the platinum distribution at different time intervals after cisplatin treatment of mice was determined quantitatively in different tissues including cochlea, testis and kidney. For this purpose, cold-curing resin blocks spiked with different amounts of platinum acetyl acetonate prior to curing were ablated after sectioning at 5 μm thickness and were analysed using ICP-MS after microwave digestion. High spatial resolution and limits of detection in the low ppb range (8 μg kg(-1)) were achieved using a simple and efficient sample preparation. External calibration using the Technovit 7100 standards proved to yield precise and reproducible quantification results. The distribution and retention behaviour of cisplatin in the organs was investigated using the new calibration method.
本文提出了一种基于冷聚合树脂（Technovit 7100）包埋组织的激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）对组织成像进行定量分析的新方法。针对顺铂的严重副作用，本研究定量测定了顺铂处理后不同时间点小鼠不同组织（包括耳蜗、睾丸和肾脏）中的铂分布。为此，在冷聚合树脂块固化前加入不同量的乙酰丙酮铂，然后将其以 5 μm 的厚度切片，用微波消解后用 ICP-MS 进行分析。采用简单高效的样品制备方法，实现了高空间分辨率和低 ppb 范围内（8 μg kg(-1))的检测限。使用 Technovit 7100 标准进行外部校准，证明可以得到精确和可重复的定量结果。新的校准方法用于研究顺铂在器官中的分布和保留行为。
