Köppen C, Reifschneider O, Castanheira I, Sperling M, Karst U, Ciarimboli G
University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 49149 Münster, Germany.
Metallomics. 2015 Dec;7(12):1595-603. doi: 10.1039/c5mt00226e. Epub 2015 Oct 19.
This work presents a quantitative bioimaging method for platinum based on laser ablation-inductively coupled plasma-mass spectrometry and its application for a biomedical study concerning toxic side effects of cisplatin. To trace the histopathology back to cisplatin, platinum was localized and quantified in major functional units of testicle, cochlea, kidney, nerve and brain sections from cisplatin treated mice. The direct consideration of the histology enables precise interpretation of the Pt images and the novel quantitative evaluation approach allows significantly more precise investigations than the pure image. For the first time, platinum was detected and quantified in all major injured structures including organ of Corti of cochlea and seminiferous tubule of testicle. In this way, proximal tubule in kidney, Leydig cells in testicle, stria vascularis and organ of Corti in cochlea and nerve fibers in sciatic nerves are confirmed as targets of cisplatin in these organs. However, the accumulation of platinum in almost all investigated structures also raises questions about more complex pathogenesis including direct and indirect interruption of several biological processes.
这项工作提出了一种基于激光烧蚀-电感耦合等离子体质谱法的铂定量生物成像方法及其在顺铂毒性副作用生物医学研究中的应用。为了将组织病理学追溯到顺铂，在顺铂处理小鼠的睾丸、耳蜗、肾脏、神经和脑切片的主要功能单元中对铂进行定位和定量。对组织学的直接考虑能够精确解释铂图像，而新颖的定量评估方法比单纯的图像能进行更精确的研究。首次在所有主要受损结构中检测和定量了铂，包括耳蜗的柯蒂氏器和睾丸的生精小管。通过这种方式，肾脏中的近端小管、睾丸中的莱迪希细胞、耳蜗中的血管纹和柯蒂氏器以及坐骨神经中的神经纤维被确认为这些器官中顺铂的作用靶点。然而，几乎所有研究结构中铂的积累也引发了关于更复杂发病机制的问题，包括对几个生物过程的直接和间接干扰。
