Dipartimento di Scienze del Farmaco, Università degli Studi di Pavia, Viale Taramelli, 14, 27100, Pavia, Italy,
Aging Clin Exp Res. 2013 Dec;25(6):711-4. doi: 10.1007/s40520-013-0099-5. Epub 2013 Aug 10.
The purpose of this study was to investigate whether the documented difficulties of physiological amounts of essential amino acids (EAAs) (7 g) to induce protein synthesis could be reflected in a simple method adaptable to a clinical setting. Sixteen healthy individuals, nine elderly (75.3 ± 3.5 years), and seven young (28 ± 2.5 years) were enrolled in the study. Five minutes before EAA ingestion (baseline) and 20, 40, 60, 90, 120, 180 min after EAA ingestion, venous blood samples were taken from the ante-cubital vein to determine the concentrations of EAAs (μmol/L). The results show that plasma EAA increases were significantly higher in old than in young persons at the considered time points (from p < 0.004 to p < 0.001) (unpaired Student t test). However, the velocity rate of the increasing was slower in old subjects than in young group. The study shows that EAAs ingestion by old subject is associated with reduced muscle EAA uptake.
本研究旨在探讨生理剂量的必需氨基酸（EAA）（7g）是否能诱导蛋白质合成，这一现象能否用一种简单的方法来反映，该方法适应于临床环境。本研究纳入了 16 名健康个体，其中 9 名老年人（75.3±3.5 岁），7 名年轻人（28±2.5 岁）。在 EAA 摄入前 5 分钟（基线）以及摄入后 20、40、60、90、120、180 分钟，从肘前静脉采集静脉血样，以测定 EAA（μmol/L）的浓度。结果表明，在考虑的时间点，老年人的血浆 EAA 增加明显高于年轻人（从 p<0.004 到 p<0.001）（未配对学生 t 检验）。然而，老年人组中 EAA 增加的速度比年轻组慢。研究表明，老年受试者摄入 EAA 与肌肉 EAA 摄取减少有关。
