Chojnacki Andrew, Weiss Samuel
Department of Cell Biology and Anatomy, Faculty of Medicine, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2013;1059:3-12. doi: 10.1007/978-1-62703-574-3_1.
The propagation of neural precursors in culture is an essential tool for the study of the signaling matrix that regulates their proliferation, self-renewal, and generation of terminally differentiated progeny. Neural precursors can be expanded in vitro using both adherent and non-adherent culture protocols. The culture of fetal human neural precursors in the absence of serum as free-floating clusters of cells termed neurospheres is described here.
