Gelati Maurizio, Profico Daniela, Projetti-Pensi Massimo, Muzi Gianmarco, Sgaravizzi Giada, Vescovi Angelo Luigi
Laboratorio Cellule Staminali, Cell Factory e Biobanca Azienda Ospedaliera Santa Maria di Terni, Terni, Italy.
Methods Mol Biol. 2013;1059:65-77. doi: 10.1007/978-1-62703-574-3_6.
NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of "clinical grade" human neural stem cell lines.
神经干细胞已被证明在移植到哺乳动物中枢神经系统中非常有用，可用于在神经退行性疾病动物模型中研究利用神经干细胞治疗神经退行性疾病。为了将中枢神经系统的细胞治疗推进到临床应用阶段，有必要建立连续、标准化、临床级（即按照良好生产规范指南生产）的人类神经干细胞系。在本章中，我们阐述了一些在我们的GMP细胞库中常规用于生产“临床级”人类神经干细胞系的方案。
