Blackmore Daniel G, Rietze Rodney L
Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia.
Methods Mol Biol. 2013;1059:183-94. doi: 10.1007/978-1-62703-574-3_16.
Since its inception in 1992 [Reynolds and Weiss, Science 255:1707-10, 1992], the neurosphere assay (NSA) has proven an exceptionally useful tool in detecting neural stem cells (NSCs) in both the developing and adult mammalian brain. To date, over 1,300 manuscripts have been published employing the assay, attesting to the robustness of the assay, and its ease of use. However, a brief survey of the literature demonstrates that the number of primary neurospheres generated from essentially the same anatomical region (i.e., the periventricular region of the rostral lateral ventricle) ranges between 150 and 936 [Gritti et al., J Neurosci 22:437-445, 2002; Tropepe et al., J Neurosci 17:7850-59, 1997; Doetsch et al., Cell 97:703-16, 1999; Enwere et al., J Neurosci 24:8354-65, 2004]. Indeed, in our hands we typically generate approximately 1,800 primary spheres when harvesting tissue from the same region.
自1992年首次出现[雷诺兹和魏斯，《科学》255:1707 - 10，1992]以来，神经球测定法（NSA）已被证明是检测发育中和成年哺乳动物大脑中神经干细胞（NSCs）的一种极其有用的工具。迄今为止，已有超过1300篇使用该测定法的手稿发表，证明了该测定法的稳健性及其易用性。然而，对文献的简要调查表明，从基本相同的解剖区域（即 Rostral外侧脑室的室周区域）产生的原代神经球数量在150至936之间[格里蒂等人，《神经科学杂志》22:437 - 445，2002；特罗佩佩等人，《神经科学杂志》17:7850 - 59，1997；多伊奇等人，《细胞》97:703 - 16，1999；恩韦雷等人，《神经科学杂志》24:8354 - 65，2004]。事实上，在我们手中，当从同一区域采集组织时，我们通常会产生大约1800个原代球体。
