Limberaki E, Eleftheriou Ph, Vagdatli E, Kostoglou V, Petrou Ch
Department of Medical Laboratory Studies, School of Health and Care Professions Alexander Technological Educational Institute of Thessaloniki, Greece.
Hippokratia. 2012 Apr;16(2):118-23.
The influence of factors such as age, sex, life style and smoking on oxidative stress status of the organism remains unclear. There is evidence that dietary intake of antioxidants is thought to enforce the organism ability to counteract free radicals. Administration of synthetic antioxidants as dietary supplements does not seem to have the same beneficial effect as consumption of the same antioxidants as part of food ingredients. This work focuses on the investigation of age and diet effects on oxidative stress and examines the hypotheses of their significant influence.
Blood samples of 146 volunteers, were collected and allocated in three age groups. All volunteers completed a questionnaire concerning home and working environmental conditions, special habits and dietary preferences. We implemented a thirty days diet rich in antioxidants in 55 volunteers. Antioxidant activity was estimated before and after the special diet by measuring the influence of serum in oxidation of ABTS by the ferryl myoglobinhydrogen peroxide system.
Our findings showed unexpected lower serum total antioxidant capacity (TAC) in younger people (ages 18-35 yrs) 79%, compared to middle aged and elderly individuals and a large increase 62% in serum TAC of all age-groups after the one-month special diet.
These results imply that a diet rich in antioxidants based on antioxidant rich food consumption and not on single antioxidants administration, can increase the antioxidant status of the organism and offer better health. The total serum antioxidant status increases with age and this fact should be taken into account when TAC is measured in different diseases.
年龄、性别、生活方式和吸烟等因素对机体氧化应激状态的影响尚不清楚。有证据表明，饮食中摄入抗氧化剂被认为可以增强机体对抗自由基的能力。作为膳食补充剂服用合成抗氧化剂似乎没有与作为食物成分摄入相同抗氧化剂产生的有益效果相同。这项工作着重研究年龄和饮食对氧化应激的影响，并检验它们具有显著影响的假设。
收集了146名志愿者的血样，并将其分为三个年龄组。所有志愿者都完成了一份关于家庭和工作环境条件、特殊习惯和饮食偏好的问卷。我们让55名志愿者进行了为期30天富含抗氧化剂的饮食。通过测量血清对高铁肌红蛋白 - 过氧化氢系统氧化ABTS的影响，在特殊饮食前后评估抗氧化活性。
我们的研究结果显示，与中年人和老年人相比，年轻人（18 - 35岁）的血清总抗氧化能力（TAC）出人意料地低79%，而在为期一个月的特殊饮食后，所有年龄组的血清TAC大幅增加了62%。
这些结果表明，基于富含抗氧化剂食物的摄入而非单一抗氧化剂的服用的富含抗氧化剂的饮食，可以提高机体的抗氧化状态并带来更好的健康。血清总抗氧化状态随年龄增长而增加，在测量不同疾病的TAC时应考虑这一事实。
