Černiauskas Linas, Mažeikienė Asta, Mazgelytė Eglė, Petrylaitė Eglė, Linkevičiūtė-Dumčė Aušra, Burokienė Neringa, Karčiauskaitė Dovilė
Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, M. K. Čiurlionio St. 21, LT-03101 Vilnius, Lithuania.
Clinics of Internal Diseases, Family Medicine and Oncology, Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, LT-03101 Vilnius, Lithuania.
Biomedicines. 2023 Sep 3;11(9):2450. doi: 10.3390/biomedicines11092450.
Cardiovascular diseases are the leading cause of mortality worldwide. Since atherosclerosis, an inflammatory, lipid-driven disease, is an underlying basis for the development of cardiovascular disease, it is important to understand its relationship with confounding factors, such as oxidative lipid degradation. In contrast, circulating antioxidants prevent oxidative lipid damage, and therefore, may be associated with reduced development of atherosclerosis. We aimed to assess oxidative lipid degradation biomarker malondialdehyde (MDA) and antioxidant defense system components, total antioxidant capacity (TAC) and superoxide dismutase (SOD) inhibition rate levels, in healthy women and evaluate their relationships with age, anthropometric measures, and lipid metabolism biomarkers. The study included 86 healthy middle-aged women. MDA in human serum samples was evaluated by HPLC, and the TAC and SOD inhibition rates were measured by photometric methods. MDA was found to be associated with age, total cholesterol, non-HDL cholesterol, apolipoprotein B and triacylglycerols. TAC was shown to be associated with age, BMI, and waist circumference, as well as lipid metabolism biomarkers apolipoprotein B and triacylglycerol, while SOD inhibition rate was only associated with total cholesterol, apolipoprotein B and triacylglycerols. In conclusion, the association of oxidative status indices, MDA, TAC and SOD, with cardiovascular risk factors suggests that they could be additional useful biomarkers in the research of aging, obesity, and atherosclerosis pathogenesis.
心血管疾病是全球范围内主要的死亡原因。由于动脉粥样硬化是一种炎症性、脂质驱动的疾病，是心血管疾病发生发展的潜在基础，因此了解其与氧化脂质降解等混杂因素的关系非常重要。相比之下，循环中的抗氧化剂可防止氧化脂质损伤，因此可能与动脉粥样硬化发展减缓有关。我们旨在评估健康女性中氧化脂质降解生物标志物丙二醛（MDA）以及抗氧化防御系统成分、总抗氧化能力（TAC）和超氧化物歧化酶（SOD）抑制率水平，并评估它们与年龄、人体测量指标和脂质代谢生物标志物的关系。该研究纳入了86名健康中年女性。通过高效液相色谱法评估人血清样本中的MDA，通过光度法测量TAC和SOD抑制率。发现MDA与年龄、总胆固醇、非高密度脂蛋白胆固醇、载脂蛋白B和甘油三酯有关。TAC与年龄、体重指数和腰围以及脂质代谢生物标志物载脂蛋白B和甘油三酯有关，而SOD抑制率仅与总胆固醇、载脂蛋白B和甘油三酯有关。总之，氧化状态指标MDA、TAC和SOD与心血管危险因素的关联表明，它们可能是衰老、肥胖和动脉粥样硬化发病机制研究中额外有用的生物标志物。
