Kountouras J, Sofianou D, Gavalas E, Sianou E, Zavos C, Meletis G, Tsiaousi E
Department of Medicine, Second Medical Clinic, Aristotle University of Thessaloniki, Ippokration Hospital, Thessaloniki, Greece.
Hippokratia. 2012 Oct;16(4):347-9.
The aim of this preliminary study was to investigate the in vitro effect of "non-antibiotic" trimebutine against reference strains Staphylococcus aureus ATCC 29213, ATCC 25923, Escherichia coli ATCC 25922, ATCC 35218, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212; microbiota that are potentially involved in the pathophysiology of post-infectious functional gastrointestinal disorders.
Trimebutine activity was assessed by the broth microdilution method according to Clinical and Laboratory Standards Institute recommendations against reference strains S. aureus ATCC 29213 and ATCC 25923, E. coli ATCC 25922 and ATCC 35218, P. aeruginosa ATCC 27853 and E. faecalis ATCC 29212. Bactericidal activity of the compound was determined by spreading a 10 μL aliquot on Mueller-Hinton agar from each dilution showing non-visible growth. All tests were carried out in triplicate.
Trimebutine was active against all strains tested presenting with MIC ranging from 1024 to 4000 mg/L. MIC and MBC were similar for E. coli ATCC 25922 and P. aeruginosa ATCC 27853 whereas for Gram-positive isolates and E. coli ATCC 35218 the MBC was higher.
We demonstrated the in vitro bacteriostatic/bactericidal activity of trimebutine against bacteria frequently colonizing the gastrointestinal tract and potentially involved in human gastrointestinal infections that might trigger post-infectious functional gastrointestinal disorders.
本初步研究旨在调查“非抗生素”曲美布汀对金黄色葡萄球菌ATCC 29213、ATCC 25923、大肠埃希菌ATCC 25922、ATCC 35218、铜绿假单胞菌ATCC 27853和粪肠球菌ATCC 29212等参考菌株的体外作用；这些微生物可能参与感染后功能性胃肠疾病的病理生理过程。
根据临床和实验室标准协会的建议，采用肉汤微量稀释法评估曲美布汀对金黄色葡萄球菌ATCC 29213和ATCC 25923、大肠埃希菌ATCC 25922和ATCC 35218、铜绿假单胞菌ATCC 27853以及粪肠球菌ATCC 29212等参考菌株的活性。通过将来自每个显示无可见生长的稀释度的10 μL等分试样铺展在穆勒-欣顿琼脂上，测定该化合物的杀菌活性。所有测试均重复进行三次。
曲美布汀对所有测试菌株均有活性，其最低抑菌浓度（MIC）范围为1024至4000 mg/L。大肠埃希菌ATCC 25922和铜绿假单胞菌ATCC 27853的MIC和最低杀菌浓度（MBC）相似，而对于革兰氏阳性分离株和大肠埃希菌ATCC 35218，MBC更高。
我们证明了曲美布汀对经常定植于胃肠道且可能参与人类胃肠道感染（可能引发感染后功能性胃肠疾病）的细菌具有体外抑菌/杀菌活性。
