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A technique for temporal bone sections using non-decalcified frozen guinea pig cochleas: a study of succinic dehydrogenases.

作者信息

Yang F S, Han J S, Dong Y, Liu D X

机构信息

Department of Anatomy, Hengyang Medical College, Hunan, People's Republic of China.

出版信息

Eur Arch Otorhinolaryngol. 1990;247(5):283-6. doi: 10.1007/BF00176537.

DOI:10.1007/BF00176537
PMID:2393561
Abstract

We have developed a technique for sectioning temporal bones and have studied non-decalcified frozen guinea pig cochleas to localize succinic dehydrogenases. The technique is simple and practicable. The 6-microns sections used preserve both fine cochlear structure and satisfactory histochemical localization for identifying the succinic dehydrogenases. The technique can also be extended to immunological test, detecting transmitters and investigating enzymes in the cochlea.

摘要

相似文献

1
A technique for temporal bone sections using non-decalcified frozen guinea pig cochleas: a study of succinic dehydrogenases.
Eur Arch Otorhinolaryngol. 1990;247(5):283-6. doi: 10.1007/BF00176537.
2
Gentamicin-induced alterations of succinic dehydrogenase activity in the organ of Corti as revealed by non-decalcified frozen sections of the guinea pig's cochlea.
Eur Arch Otorhinolaryngol. 1991;248(4):195-201. doi: 10.1007/BF00173656.
3
[Succinic and lactic dehydrogenases of the cochlea--histochemical determination and the effect of sound].
Nihon Jibiinkoka Gakkai Kaiho. 1969 Jun;72(6):1130-55.
4
A comparative immunofluorescent study of fixed decalcified tissue and frozen non-decalcified tissue from the guinea pig cochlea.豚鼠耳蜗固定脱钙组织与冷冻未脱钙组织的比较免疫荧光研究。
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7
[Observation of localization of succinic dehydrogenase in the guinea pig cochlea].
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8
The effect of frusemide, piretanide and bumetanide on cochlear succinic dehydrogenase.速尿、吡咯他尼和布美他尼对耳蜗琥珀酸脱氢酶的作用。
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9
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10
The use of immunofluorescence in the non-decalcified frozen guinea pig cochlea to detect autoantibodies in inner ear disorders.免疫荧光法在未脱钙的豚鼠冷冻耳蜗中用于检测内耳疾病中的自身抗体。
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引用本文的文献

1
Gentamicin-induced alterations of succinic dehydrogenase activity in the organ of Corti as revealed by non-decalcified frozen sections of the guinea pig's cochlea.
Eur Arch Otorhinolaryngol. 1991;248(4):195-201. doi: 10.1007/BF00173656.

本文引用的文献

1
Some oxidizing enzymes in the cochlea.
Ann Otol Rhinol Laryngol. 1962 Mar;71:96-104. doi: 10.1177/000348946207100108.
2
DISTRIBUTION OF SOME OXIDIZING ENZYMES IN THE COCHLEA.
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METABOLIC PATHWAYS IN THE VESTIBULAR LABYRINTH AS REVEALED BY HISTOCHEMICAL TECHNICS.组织化学技术揭示的前庭迷路中的代谢途径
Acta Otolaryngol. 1964 May;57:484-92. doi: 10.3109/00016486409137110.
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HISTOCHEMICAL LOCALIZATION OF DEHYDROGENASES IN THE COCHLEA OF LIVING ANIMALS.
脱氢酶在活体动物耳蜗中的组织化学定位
Laryngoscope. 1963 Aug;73:1061-83. doi: 10.1288/00005537-196308000-00005.
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Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole.使用一种新的对硝基苯基取代的双四氮唑对琥珀酸脱氢酶进行细胞化学显示。
J Histochem Cytochem. 1957 Jul;5(4):420-36. doi: 10.1177/5.4.420.
6
Frozen-section autoradiography of the cochlea: evidence for differential distribution of amino acids.耳蜗的冰冻切片放射自显影术：氨基酸差异分布的证据
Ann Otol Rhinol Laryngol. 1983 May-Jun;92(3 Pt 1):254-8. doi: 10.1177/000348948309200308.
7
The use of immunofluorescence in the non-decalcified frozen guinea pig cochlea to detect autoantibodies in inner ear disorders.免疫荧光法在未脱钙的豚鼠冷冻耳蜗中用于检测内耳疾病中的自身抗体。
Arch Otorhinolaryngol. 1987;244(4):241-5. doi: 10.1007/BF00455313.
8
An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea.
J Laryngol Otol. 1988 Mar;102(3):215-8. doi: 10.1017/s0022215100104554.
9
A comparative immunofluorescent study of fixed decalcified tissue and frozen non-decalcified tissue from the guinea pig cochlea.豚鼠耳蜗固定脱钙组织与冷冻未脱钙组织的比较免疫荧光研究。
Arch Otorhinolaryngol. 1988;244(6):337-41. doi: 10.1007/BF00497460.