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An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea.

作者信息

Soliman A M

机构信息

Department of Otorhinolaryngology, University of Tübingen, Germany.

出版信息

J Laryngol Otol. 1988 Mar;102(3):215-8. doi: 10.1017/s0022215100104554.

DOI:10.1017/s0022215100104554
PMID:3282016
Abstract

A technique for producing frozen sections from the non-decalcified guinea pig cochlea is described. The sections can be utilised for various immunofluorescent studies, including the detection of humoral antibodies against different cellular elements in patients with cochleo-vestibular disorders. The technique assures the preservation of the cochlear architecture and the tissue antigenicity, and it avoids damage to the fine cochlear structural details.

摘要

相似文献

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An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea.
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引用本文的文献

1
Optimizing immunofluorescence for the testing of autoantibodies in inner ear disorders.
Arch Otorhinolaryngol. 1988;245(1):28-35. doi: 10.1007/BF00463545.
2
A comparative immunofluorescent study of fixed decalcified tissue and frozen non-decalcified tissue from the guinea pig cochlea.豚鼠耳蜗固定脱钙组织与冷冻未脱钙组织的比较免疫荧光研究。
Arch Otorhinolaryngol. 1988;244(6):337-41. doi: 10.1007/BF00497460.
3
A technique for temporal bone sections using non-decalcified frozen guinea pig cochleas: a study of succinic dehydrogenases.
Eur Arch Otorhinolaryngol. 1990;247(5):283-6. doi: 10.1007/BF00176537.
4
Inner ear fine structures of the hamster in frozen sections used for immunohistochemical assays for inner ear diseases.用于内耳疾病免疫组织化学检测的仓鼠内耳冰冻切片的精细结构。
Eur Arch Otorhinolaryngol. 1991;248(4):250-2. doi: 10.1007/BF00173666.