Soliman A M
Department of Otolaryngology, University of Tübingen, Federal Republic of Germany.
Arch Otorhinolaryngol. 1988;244(6):337-41. doi: 10.1007/BF00497460.
The immunofluorescence test (IF) is an important diagnostic laboratory tool for detecting immune-mediated sensorineural hearing loss (SNHL). The results of applying the IF to ethanol-fixed, paraffin-embedded, decalcified sections of guinea pig cochlea as compared with frozen non-decalcified preparations are presented. Sera from ten patients with SNHL were tested in addition to antibody-positive and negative human serum controls. Despite fixation and prolonged decalcification, deparaffinized cochlear sections showed a strong positive reaction with most of the positive serum controls. Anti-endothelial antibodies were detected in the serum of one patient and anti-nuclear antibodies in another. The fine cochlear structure was clearly delineated. However, the decalcified specimens failed to react with one serum model, which revealed a positive fluorescence when incubated with the frozen non-decalcified cochlear preparations. These latter findings denote possible leaching of the antigenic material from the sections. This study further shows that frozen nondecalcified cochlear tissue is a reliable tissue source for the interpretation of the results obtained with IF.
免疫荧光试验（IF）是检测免疫介导的感音神经性听力损失（SNHL）的一项重要诊断实验室工具。本文展示了将IF应用于乙醇固定、石蜡包埋、脱钙的豚鼠耳蜗切片，并与冷冻未脱钙制剂进行比较的结果。除了抗体阳性和阴性的人血清对照外，还检测了10例SNHL患者的血清。尽管经过固定和长时间脱钙处理，但脱蜡后的耳蜗切片与大多数阳性血清对照仍呈现出强阳性反应。在1例患者血清中检测到抗内皮抗体，在另1例患者血清中检测到抗核抗体。耳蜗的精细结构清晰可辨。然而，脱钙标本与一种血清模型未发生反应，而该血清模型与冷冻未脱钙的耳蜗制剂孵育时显示出阳性荧光。后一项发现表明可能存在抗原物质从切片中浸出的情况。本研究进一步表明，冷冻未脱钙的耳蜗组织是用于解释IF所得结果的可靠组织来源。
