Nair Hari, Woo Fred, Hoofnagle Andrew N, Baird Geoffrey S
Department of Laboratory Medicine, University of Washington, P.O. Box 357110, 1959 NE Pacific Street, Seattle, WA 98185, USA.
J Toxicol. 2013;2013:329407. doi: 10.1155/2013/329407. Epub 2013 Jul 10.
An important role of the clinical toxicology laboratory is to provide continuous diagnostic testing for patients with altered mental status and for other medical indications. To meet these needs, we have developed a new Gas Chromatography-Mass Spectrometry (GC-MS) platform that facilitates routine screening and automated reporting of 212 drugs by laboratory technologists around the clock without the need to sign out by an on-site mass spectrometry-trained toxicologist. The platform uses a programmable temperature vaporizer (PTV) injector for large sample volume injection and the free software Automated Mass Spectral Deconvolution and Identification System (AMDIS) for data reduction and spectral matching that facilitates rapid library searching and analyte identification. Method comparison with 118 patient samples demonstrated that this platform and data searching algorithm independently provided improvements in sensitivity compared to an established GC-MS platform. Further examination of the role of the data processing software and the in-house databases used in the established versus the new platform demonstrated that the improved analytical sensitivity of the new platform was attributed to both the technical superiority of the new GC-MS instrumentation and the use of AMDIS in conjunction with the newly generated in-house library for data processing.
临床毒理学实验室的一个重要作用是为精神状态改变的患者以及其他医学指征提供持续的诊断检测。为满足这些需求，我们开发了一个新的气相色谱 - 质谱联用（GC-MS）平台，该平台便于实验室技术人员全天候对212种药物进行常规筛查和自动报告，无需经过现场受过质谱分析培训的毒理学家签字确认。该平台使用可编程温度汽化器（PTV）进样器进行大体积样品进样，并使用免费软件自动质谱解卷积和鉴定系统（AMDIS）进行数据简化和光谱匹配，这有助于快速进行库检索和分析物鉴定。与118份患者样品的方法比较表明，与已建立的GC-MS平台相比，该平台和数据搜索算法独立地提高了灵敏度。对已建立平台与新平台中使用的数据处理软件和内部数据库的作用进行进一步研究表明，新平台分析灵敏度的提高归因于新GC-MS仪器的技术优势以及AMDIS与新生成的内部库结合用于数据处理。
