在人神经母细胞瘤细胞系中鉴定一种因子 XIII A 变体。
Identification of a FXIIIA variant in human neuroblastoma cell lines.
作者信息
lannaccone Martina, Giuberti Gaia, De Vivo Giulia, Caraglia Michele, Gentile Vittorio
机构信息
Department of Biochemistry, Biophysics and General Pathology, Second University of Naples 80138 Naples, Italy.
出版信息
Int J Biochem Mol Biol. 2013 Jul 29;4(2):102-7. Print 2013.
Abstract
FXIII is a transglutaminase consisting of two catalytic (FXIIIA) and two non-catalytic subunits (FXIIIB) in plasma, where this enzyme is responsible for stabilizing fibrin clots. Although possible functions of intracellular FXIIIA have been proposed, these remain to be established. We show that a 40 kDa protein species of FXIIIA is present in the human neuroblastoma cell lines SH-SY5Y and LAN5. These data reveal the presence of a new uncharacterised variant of FXIIIA, possibly due to an alternative splicing, in nervous cells.
摘要
因子 XIII 是一种转谷氨酰胺酶，在血浆中由两个催化亚基（因子 XIIIA）和两个非催化亚基（因子 XIIIB）组成，该酶负责稳定纤维蛋白凝块。尽管已经提出了细胞内因子 XIIIA 的可能功能，但这些功能仍有待确定。我们发现，在人神经母细胞瘤细胞系 SH-SY5Y 和 LAN5 中存在一种 40 kDa 的因子 XIIIA 蛋白。这些数据揭示了神经细胞中存在一种新的未表征的因子 XIIIA 变体，可能是由于选择性剪接所致。
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