Achyuthan K E, Rowland T C, Birckbichler P J, Lee K N, Bishop P D, Achyuthan A M
ZymeTx, Inc., Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Mol Cell Biochem. 1996 Sep 6;162(1):43-9. doi: 10.1007/BF00250994.
The affinities of Factor XIII (FXIII), Factor XIIIa (FXIIIa), and cellular transglutaminase (Tg) for fibrinogen (Fgn), fibrin (Fbn), and fibronectin (Fn) were compared using a solid-phase binding assay. Initial rates of binding were as follows: FXIII bound Fbn 3-fold more than Fgn. FXIII did not bind Fn till 20 min. Increasing the ligands concentrations and binding time, resulted in weak binding of FXIII to Fn. FXIIIa bound Fbn 2-fold more than Fgn and 28-fold more than Fn. Tg bound Fn approximately 130-fold more than either Fgn or Fbn. At equilibrium, the extent of binding was determined to be as follows: FXIII bound Fbn 3-15-fold more than Fgn and 8-fold more than Fn. FXIIIa bound Fgn and Fbn equally and 12-25-fold more than Fn. FXIIIa bound Fgn or Fbn 2-fold and 25-fold greater than FXIII-Fbn and FXIII-Fgn interactions, respectively. Tg bound about equally to Fgn and Fbn and 10-20-fold less than Fn. The Kds' for FXIIIa binding to Fn, Fgn, and Fbn were 100, 23, and 19 nM, respectively. The Kd for Tg binding to Fn was 6.5 nM. The binding hierarchies are: [Tg-Fn] > [FXIIIa-Fgn] = [FXIIIa-Fbn] > [FXIII-Fbn] > [FXIII-Fgn] = [FXIIIa-Fn] > [Tg-Fbn] = [Tg-Fgn] > [FXIII-Fn]. Such hierarchies could regulate the cross-linkings by FXIIIa and Tg during hemostasis, wound healing, and cell adhesion.
使用固相结合试验比较了因子 XIII（FXIII）、因子 XIIIa（FXIIIa）和细胞转谷氨酰胺酶（Tg）对纤维蛋白原（Fgn）、纤维蛋白（Fbn）和纤连蛋白（Fn）的亲和力。结合的初始速率如下：FXIII 与 Fbn 的结合量是与 Fgn 结合量的 3 倍。FXIII 在 20 分钟前不与 Fn 结合。增加配体浓度和结合时间，导致 FXIII 与 Fn 的弱结合。FXIIIa 与 Fbn 的结合量是与 Fgn 结合量的 2 倍，是与 Fn 结合量的 28 倍。Tg 与 Fn 的结合量比与 Fgn 或 Fbn 的结合量大约多 130 倍。在平衡状态下，结合程度如下：FXIII 与 Fbn 的结合量比与 Fgn 的结合量多 3 - 15 倍，比与 Fn 的结合量多 8 倍。FXIIIa 与 Fgn 和 Fbn 的结合量相等，比与 Fn 的结合量多 12 - 25 倍。FXIIIa 与 Fgn 或 Fbn 的结合量分别比 FXIII - Fbn 和 FXIII - Fgn 相互作用大 2 倍和 25 倍。Tg 与 Fgn 和 Fbn 的结合量大致相等，比与 Fn 的结合量少 10 - 20 倍。FXIIIa 与 Fn、Fgn 和 Fbn 结合的解离常数（Kds）分别为 100、23 和 19 nM。Tg 与 Fn 结合的 Kd 为 6.5 nM。结合层次为：[Tg - Fn] > [FXIIIa - Fgn] = [FXIIIa - Fbn] > [FXIII - Fbn] > [FXIII - Fgn] = [FXIIIa - Fn] > [Tg - Fbn] = [Tg - Fgn] > [FXIII - Fn]。这样的层次结构可以在止血、伤口愈合和细胞黏附过程中调节 FXIIIa 和 Tg 的交联作用。
