Department of Chemistry and Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Biomed Res Int. 2013;2013:287638. doi: 10.1155/2013/287638. Epub 2013 Jul 11.
The biosynthesis of nanoparticles has been proposed as a cost effective environmental friendly alternative to chemical and physical methods. Microbial synthesis of nanoparticles is under exploration due to wide biomedical applications, research interest in nanotechnology and microbial biotechnology. In the present study, an ecofriendly process for the synthesis of nanoparticles using a novel Nocardiopsis sp. MBRC-1 has been attempted. We used culture supernatant of Nocardiopsis sp. MBRC-1 for the simple and cost effective green synthesis of silver nanoparticles. The reduction of silver ions occurred when silver nitrate solution was treated with the Nocardiopsis sp. MBRC-1 culture supernatant at room temperature. The nanoparticles were characterized by UV-visible, TEM, FE-SEM, EDX, FTIR, and XRD spectroscopy. The nanoparticles exhibited an absorption peak around 420 nm, a characteristic surface plasmon resonance band of silver nanoparticles. They were spherical in shape with an average particle size of 45 ± 0.15 nm. The EDX analysis showed the presence of elemental silver signal in the synthesized nanoparticles. The FTIR analysis revealed that the protein component in the form of enzyme nitrate reductase produced by the isolate in the culture supernatant may be responsible for reduction and as capping agents. The XRD spectrum showed the characteristic Bragg peaks of 1 2 3, 2 0 4, 0 4 3, 1 4 4, and 3 1 1 facets of the face centered cubic silver nanoparticles and confirms that these nanoparticles are crystalline in nature. The prepared silver nanoparticles exhibited strong antimicrobial activity against bacteria and fungi. Cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) showed a dose-response activity. IC50 value was found to be 200 μg/mL of AgNPs against HeLa cancer cells. Further studies are needed to elucidate the toxicity and the mechanism involved with antimicrobial and anticancer activity of the synthesized AgNPs as nanomedicine.
纳米粒子的生物合成被认为是一种具有成本效益且对环境友好的替代化学和物理方法的方法。由于广泛的生物医学应用、纳米技术和微生物生物技术的研究兴趣，微生物合成纳米粒子正在被探索。在本研究中，尝试使用一种新型诺卡氏菌 MBRC-1 来进行纳米粒子的生物合成。我们使用诺卡氏菌 MBRC-1 的培养上清液来简单且经济有效地绿色合成银纳米粒子。当硝酸银溶液与诺卡氏菌 MBRC-1 培养上清液在室温下处理时，银离子发生还原。通过 UV-可见光谱、TEM、FE-SEM、EDX、FTIR 和 XRD 光谱对纳米粒子进行了表征。纳米粒子在 420nm 左右表现出吸收峰，这是银纳米粒子的特征表面等离子体共振带。它们呈球形，平均粒径为 45±0.15nm。EDX 分析表明，合成的纳米粒子中存在元素银信号。FTIR 分析表明，分离物在培养上清液中产生的酶硝酸盐还原酶的蛋白质成分可能负责还原和作为封端剂。XRD 图谱显示了面心立方银纳米粒子的特征布拉格峰 1 2 3、2 0 4、0 4 3、1 4 4 和 3 1 1 面，证实这些纳米粒子是晶态的。所制备的银纳米粒子对细菌和真菌表现出很强的抗菌活性。生物合成的 AgNPs 对体外人宫颈癌细胞系（HeLa）的细胞毒性显示出剂量反应活性。发现 AgNPs 对 HeLa 癌细胞的 IC50 值为 200μg/mL。需要进一步研究来阐明所合成的 AgNPs 作为纳米药物的抗菌和抗癌活性涉及的毒性和机制。
