iTRAQ 蛋白质组学揭示骨关节炎的新型生物标志物。
iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis.
机构信息
Department of Orthopaedic Surgery, Fujita Health University Hospital , Toyoake, Aichi , Japan and.
出版信息
Biomarkers. 2013 Nov;18(7):565-72. doi: 10.3109/1354750X.2013.810667. Epub 2013 Aug 12.
OBJECTIVE
We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA).
METHODS
Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot.
RESULTS
Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA.
CONCLUSIONS
Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA.
目的
我们采用同位素相对标记与绝对定量（iTRAQ）方法对关节软骨进行全面的蛋白质组学分析，并寻找骨关节炎（OA）的候选生物标志物。
方法
从 OA 患者和股骨颈骨折对照组患者的关节软骨中采集样本。采用 iTRAQ 方法检测分子变化，并通过 Western blot 进行定量分析。
结果
通过 iTRAQ 方法，我们在 OA 患者和对照组中鉴定出 76 种具有不同表达水平的蛋白质。在这些蛋白质中，我们选择了 LECT2（白细胞衍生趋化因子-2）、BAALC（脑和急性白血病，细胞质）和 PRDX6（过氧化物酶-6），它们尚未被报道为 OA 的生物标志物。
结论
这些蛋白质与传统的 OA 生物标志物联合使用可能更好地反映 OA 的严重程度和预后。
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