Charalampakis Georgios, Abrahamsson Ingemar, Carcuac Olivier, Dahlén Gunnar, Berglundh Tord
Department of Oral Microbiology and Immunology, Institute of Odontology, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
Clin Oral Implants Res. 2014 Sep;25(9):1094-8. doi: 10.1111/clr.12235. Epub 2013 Aug 13.
To analyze the microbial profile around teeth and implants following ligature removal in experimental periodontitis and peri-implantitis in dogs.
Four implants with similar geometry and with two different surface characteristics (implant A: turned/implant B: TiUnite; NobelBiocare AB) were placed pairwise in the right side of the mandible 3 months after tooth extraction in five dogs. Experimental periodontitis and peri-implantitis were initiated 3 months later by ligature placement around implants and mandibular premolars and plaque formation. The ligatures were removed after 10 weeks. Microbial samples were obtained using paper points immediately after ligature removal, at 10 and 25 weeks after ligature removal. The microbiological analysis was performed by "checkerboard" DNA-DNA hybridization, including a panel of 16 bacterial species.
The amount of bone loss that occurred during the period following ligature removal was significantly larger at implants with a modified surface than at implants with a turned surface and at teeth. The microbiological analysis revealed that the total bacterial load increased during the period following ligature removal and established an anaerobic Gram-negative microflora.
It is suggested that the large variation in regard to the microbial profiles makes interpretation of a correlation between disease progression and microbial profiles difficult.
分析结扎去除后实验性牙周炎和种植体周围炎犬牙齿和种植体周围的微生物特征。
在5只犬拔牙3个月后，将4枚具有相似几何形状和两种不同表面特性的种植体（种植体A：车削表面/种植体B：钛易耐表面；诺贝尔生物保健公司）成对植入下颌右侧。3个月后，通过在种植体和下颌前磨牙周围结扎及形成菌斑引发实验性牙周炎和种植体周围炎。10周后去除结扎线。在去除结扎线后即刻、去除结扎线后10周和25周，使用纸尖获取微生物样本。通过“棋盘式”DNA-DNA杂交进行微生物分析，包括一组16种细菌。
去除结扎线后的时期内，具有改良表面的种植体处发生的骨吸收量显著大于具有车削表面的种植体和牙齿处。微生物分析显示，去除结扎线后的时期内总细菌载量增加，并形成了厌氧革兰氏阴性微生物群落。
提示微生物特征的巨大差异使得难以解释疾病进展与微生物特征之间的相关性。
