Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Diagn Microbiol Infect Dis. 2013 Oct;77(2):91-5. doi: 10.1016/j.diagmicrobio.2013.06.028. Epub 2013 Aug 9.
Escherichia albertii is a newly emerging enteric pathogen that has been associated with sporadic infections among humans and birds. Selected coliform isolates were screened for allelic variation in 2 housekeeping genes (lysP and mdh) specific for E. albertii. The 48 strains that were identified as E. albertii were tested for 15 virulence markers and biochemical and serogical properties. All E. albertii strains were non-motile, fermented D-glucose (with gas), D-mannitol, and D-mannose, but failed to ferment lactose and other sugars. Variable positive reactions were noted for other tests. Most strains were rough or failed to agglutinate with Shigella boydii 13 antisera and E. coli antisera with few exceptions. All strains were positive for the eaeA gene, and variable numbers were positive for the cdtB, phoE, ehxA, and stx2f genes. Results illustrate the variability extent within this lineage and highlight the importance of accurately distinguishing it within the genus Escherichia and including information within commercial databases to improve their identification.
阿尔伯蒂埃希氏菌是一种新出现的肠道病原体，与人类和鸟类的散发性感染有关。对选定的大肠埃希氏菌进行了 2 种看家基因（lysP 和 mdh）的等位基因变异筛选，这些基因是特定于阿尔伯蒂埃希氏菌的。确定为阿尔伯蒂埃希氏菌的 48 株菌被检测了 15 种毒力标记物以及生化和血清学特性。所有的阿尔伯蒂埃希氏菌菌株均不运动，发酵 D-葡萄糖（产气）、D-甘露醇和 D-甘露糖，但不能发酵乳糖和其他糖。其他测试的阳性反应存在差异。大多数菌株与志贺氏菌 13 抗血清和大肠杆菌抗血清呈粗糙或不凝集，只有少数例外。所有菌株均为 eaeA 基因阳性，而 cdtB、phoE、ehxA 和 stx2f 基因的阳性数则有所不同。结果说明了该谱系内的变异性程度，并强调了在属内准确区分它的重要性，包括在商业数据库中包含信息以改善其鉴定。
