Kowal-Vern A, Walloch J, Chou P, Gonzalez-Crussi F, Price J, Potocki D, Herman C
Loyola University Medical Center, Department of Pathology, Maywood, Illinois.
Mod Pathol. 1992 Jan;5(1):56-60.
Ewing's sarcoma is the second most common bone tumor in childhood, with an overall 5-yr survival of 40%. It is one of the poorly differentiated small spherical cell tumors frequently requiring distinction from rhabdomyosarcoma, neuroblastoma, osteosarcoma, primitive neuroectodermal tumor, and lymphoma. The majority of rhabdomyosarcomas, neuroblastomas, and osteosarcomas are aneuploid, whereas Ewing's sarcomas are usually diploid. To determine whether there is any correlation between DNA content, morphology, site, and survival in Ewing's sarcoma and extraosseous Ewing's sarcoma, 21 tumor samples were studied retrospectively (3 extraosseous Ewing's and 18 Ewing's sarcomas). The DNA analysis was performed on disaggregated paraffin-embedded tissue nuclei by flow (FCM) and image (IC) cytometry and correlated with the histology and clinical history. The DNA ploidy by FCM on 17 of 18 Ewing's sarcoma samples was 12 diploid, 1 aneuploid, and 4 tetraploid. By IC, the DNA ploidy on 16 samples was 13 diploid, 1 aneuploid, and 2 tetraploid. Three samples were nonevaluable (1 by FCM and 2 by IC). The agreement between FCM and IC was 12 of 16 (75%). The extraosseous Ewing's sarcoma tumors were 2 diploid and 1 aneuploid by IC. In this study there was no correlation between the DNA ploidy and either the histology, site, or survival.
尤因肉瘤是儿童期第二常见的骨肿瘤，总体5年生存率为40%。它是一种低分化的小圆形细胞瘤，常常需要与横纹肌肉瘤、神经母细胞瘤、骨肉瘤、原始神经外胚层肿瘤和淋巴瘤相鉴别。大多数横纹肌肉瘤、神经母细胞瘤和骨肉瘤是异倍体，而尤因肉瘤通常是二倍体。为了确定尤因肉瘤和骨外尤因肉瘤的DNA含量、形态、部位与生存率之间是否存在任何相关性，对21个肿瘤样本进行了回顾性研究（3例骨外尤因肉瘤和18例尤因肉瘤）。通过流式细胞术（FCM）和图像细胞术（IC）对石蜡包埋组织细胞核进行DNA分析，并将其与组织学和临床病史相关联。18例尤因肉瘤样本中，17例通过FCM检测的DNA倍性为12例二倍体、1例异倍体和4例四倍体。通过IC检测，16个样本的DNA倍性为13例二倍体、1例异倍体和2例四倍体。3个样本无法评估（1例通过FCM，2例通过IC）。FCM和IC之间的一致性为16例中的12例（75%）。通过IC检测，骨外尤因肉瘤肿瘤为2例二倍体和1例异倍体。在本研究中，DNA倍性与组织学、部位或生存率之间均无相关性。
