Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Opt Lett. 2013 May 15;38(10):1697-9. doi: 10.1364/OL.38.001697.
We present a fast and flexible fluorescence lifetime imaging microscopy which uses a two-dimensional acousto-optic deflector to achieve fast beam scanning across the sample and provides random access to the regions of interests (ROI). Experimental results using standard fluorescent dye and biological samples show that this system can make addressable fluorescence lifetime measurements and perform fast and flexible fluorescence lifetime imaging particularly to the discontinuous ROI in the sample.
我们提出了一种快速灵活的荧光寿命成像显微镜,它使用二维声光偏转器实现快速光束扫描整个样品,并能对感兴趣区域(ROI)进行随机访问。使用标准荧光染料和生物样品的实验结果表明,该系统可以进行可寻址的荧光寿命测量,并能快速灵活地进行荧光寿命成像,特别是针对样品中不连续的 ROI。