基于声光偏转器和空间光调制器的超快、大视场多光子显微镜。
Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator.
机构信息
College of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
出版信息
Opt Lett. 2012 Jul 1;37(13):2532-4. doi: 10.1364/OL.37.002532.
Abstract
We present an ultrafast, large-field multiphoton excitation fluorescence microscope with high lateral and axial resolutions based on a two-dimensional (2-D) acousto-optical deflector (AOD) scanner and spatial light modulator (SLM). When a phase-only SLM is used to shape the near-infrared light from a mode-locked titanium:sapphire laser into a multifocus array including the 0-order beam, a 136 μm × 136 μm field of view is achieved with a 60× objective using a 2-D AOD scanner without any mechanical scan element. The two-photon fluorescence image of a neuronal network that was obtained using this system demonstrates that our microscopy permits observation of dynamic biological events in a large field with high-temporal and -spatial resolution.
摘要
我们展示了一种基于二维（2D）声光偏转器（AOD）扫描仪和空间光调制器（SLM）的超快、大视场多光子激发荧光显微镜，具有高横向和轴向分辨率。当使用纯相位空间光调制器将锁模钛宝石激光器的近红外光整形为包括零阶光束的多焦点阵列时，使用二维 AOD 扫描仪在没有任何机械扫描元件的情况下，可在 60×物镜下实现 136μm×136μm 的视场。使用该系统获得的神经网络的双光子荧光图像表明，我们的显微镜可以在大视场中以高时空分辨率观察动态生物事件。
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