Instituto Nacional de Salud, Bogotá, Colombia.
Rev Panam Salud Publica. 2013 Jun;33(6):422-6.
To determine the genetic relationship between Streptococcus pneumoniae serotype 1 Colombian isolates recovered from invasive disease between 1994 and 2011 and recognized serotype 1 international clones.
A total of 135 S. pneumoniae serotype 1 isolates with epidemiological and antimicrobial susceptibility data (Clinical and Laboratory Standards Institute, 2012) were studied. The genetic relationship with recognized international clones was established by pulsed-field gel electrophoresis (PFGE) with SmaI restriction enzyme. Multilocus sequence typing (MLST) was standardized to determine the sequence type (ST) in seven isolates representing different clonal groups. Control and reference strain R6, and clones Sweden¹ ST217, Sweden¹ ST304, Sweden¹ ST306, and USA¹ ST615, were used.
PFGE revealed that 89.7% of the isolates were associated with Sweden¹ ST306, 3.7% were associated with Sweden¹ ST304, and 6.6% were not clonally related. Using MLST, ST306 was confirmed in six isolates and ST304 in one.
In contrast to Brazil and the United States, where clones Sweden¹ ST304 and ST227 prevail, invasive disease caused by S. pneumoniae serotype 1 in Colombia is principally associated with the dispersion of isolates related to clone Sweden¹ ST306.
确定 1994 年至 2011 年间分离的侵袭性疾病的哥伦比亚 1 型肺炎链球菌分离株与已识别的 1 型国际克隆株之间的遗传关系。
共研究了 135 株具有流行病学和抗菌药物敏感性数据的 1 型肺炎链球菌分离株（临床和实验室标准协会，2012 年）。通过 SmaI 限制酶脉冲场凝胶电泳（PFGE）确定与公认的国际克隆株的遗传关系。标准化多位点序列分型（MLST）以确定 7 株不同克隆群代表的序列型（ST）。使用对照和参考菌株 R6 以及瑞典克隆株 1 型 ST217、瑞典克隆株 1 型 ST304、瑞典克隆株 1 型 ST306 和美国克隆株 1 型 ST615。
PFGE 显示 89.7%的分离株与瑞典克隆株 1 型 ST306 相关，3.7%与瑞典克隆株 1 型 ST304 相关，6.6%与克隆株不相关。使用 MLST，在 6 株分离株中证实了 ST306，在 1 株分离株中证实了 ST304。
与巴西和美国不同，巴西和美国主要流行瑞典克隆株 1 型 ST304 和 ST227，而哥伦比亚侵袭性 1 型肺炎链球菌疾病主要与相关分离株的传播有关克隆瑞典 1 型 ST306。
