Grupo de Microbiología. Instituto Nacional de Salud, Avenida calle 26 No. 51-20, Bogotá, Colombia.
Grupo de Microbiología. Instituto Nacional de Salud, Avenida calle 26 No. 51-20, Bogotá, Colombia.
Vaccine. 2014 Feb 7;32(7):755-8. doi: 10.1016/j.vaccine.2013.12.024. Epub 2013 Dec 25.
The aim of this study was to determine the molecular characterization of invasive penicillin non-susceptible Streptococcus pneumoniae serotype 19A isolates, collected in Colombia between 1994 and 2012. A total of 115 isolates serotype 19A were analyzed. Genetic relationship of 80 isolates with minimal inhibitory concentration (MIC) to penicillin ≥0.125 μg/was determined by pulsed-field gel electrophoresis (PFGE) and selected strains were studied by multilocus sequence typing (MLST). Among the 115 isolates, resistance to penicillin in meningitis was 64.2%, in non-meningitis 32.2% were intermediate and 1.1% were high resistance. The most frequent sequence types were ST320 (33.7%), ST276 (21.5%), and ST1118 (11.2%). Five isolates were associated with the Spain(9V)-ST156 clone, and two isolates were related to Colombia(23F)-ST338 clone. S. pneumoniae serotype 19A increased in Colombia was associated with the spread of isolates genetically related to ST320 and ST276, and emergence of capsular variants of worldwide-disseminated clones.
本研究旨在确定 1994 年至 2012 年间在哥伦比亚收集的青霉素不敏感侵袭性肺炎链球菌 19A 血清型分离株的分子特征。共分析了 115 株 19A 血清型分离株。采用脉冲场凝胶电泳（PFGE）确定 80 株最小抑菌浓度（MIC）对青霉素≥0.125μg/ml 的分离株的遗传关系，并对选定的菌株进行多位点序列分型（MLST）研究。在 115 株分离株中，脑膜炎分离株对青霉素的耐药率为 64.2%，非脑膜炎分离株中，中介耐药率为 32.2%，高度耐药率为 1.1%。最常见的序列类型为 ST320（33.7%）、ST276（21.5%）和 ST1118（11.2%）。5 株与西班牙（9V）-ST156 克隆有关，2 株与哥伦比亚（23F）-ST338 克隆有关。在哥伦比亚，19A 血清型肺炎链球菌的增加与遗传上与 ST320 和 ST276 相关的分离株的传播以及全球传播克隆的荚膜变异体的出现有关。
