Palacios Paola Andrea, Duarte Carolina, Sanabria Olga, Moreno Jaime
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
Biomedica. 2017 Sep 1;37(3):390-396. doi: 10.7705/biomedica.v37i3.3223.
A total of 192 invasive Streptococcus pneumoniae isolates, from serotypes 11A, 15B/C and 23A (not included in the conjugated vaccines), were collected in Colombia between 1994 and 2014 as part of the activities of the Network surveillance system for the causative agents of pneumonia and meningitis (SIREVA II).
To determine the molecular characteristics of invasive S. pneumoniae isolates from serotypes 11A, 15B/C and 23A in Colombia from 1994 to 2014.
The molecular characterization of the isolates was carried out through Pulse-Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST).
Serotype 11A showed one clonal group represented by ST62. Serotype 15B/C was composed of three groups associated with Netherlands15B-37 ST199 (28.75%), ST8495 (18.75%), and SLV (Single-Locus Variant) of ST193 (21.25%). Isolates from serotype 23A were gathered in three clonal groups, with 70.21% closely related to ST42, 17.02% to Colombia23F-ST338, and 6.38% to Netherlands15B-37 ST199.
Clones Colombia23F-ST338 and Netherlands15B-ST199 covered more serotypes than those previously found by other authors, including serotype 23A. These analyses reveal the importance of capsular switching in the spreading of successful clones among non-vaccine serotypes causing invasive pneumococcal disease.
作为肺炎和脑膜炎病原体网络监测系统（SIREVA II）活动的一部分，1994年至2014年间在哥伦比亚收集了总共192株侵袭性肺炎链球菌分离株，这些分离株来自血清型11A、15B/C和23A（未包含在结合疫苗中）。
确定1994年至2014年间哥伦比亚血清型11A、15B/C和23A的侵袭性肺炎链球菌分离株的分子特征。
通过脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）对分离株进行分子特征分析。
血清型11A显示出一个以ST62为代表的克隆群。血清型15B/C由三组组成，分别与荷兰15B - 37 ST199（28.75%）、ST8495（18.75%）以及ST193的单一位点变体（SLV，Single-Locus Variant）（21.25%）相关。血清型23A的分离株聚集在三个克隆群中，70.21%与ST42密切相关，17.02%与哥伦比亚23F - ST338相关，6.38%与荷兰15B - 37 ST199相关。
克隆株哥伦比亚23F - ST338和荷兰15B - ST199覆盖的血清型比其他作者先前发现的更多，包括血清型23A。这些分析揭示了荚膜转换在导致侵袭性肺炎球菌疾病的非疫苗血清型中成功克隆株传播中的重要性。
