Yang Xiaojing, Cheng Lei, Li Mei, Shi Hui, Ren Hanru, Ding Zongmei, Liu Fang, Wang Yuchan, Cheng Chun
Department of Immunology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China; Department of Oncology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
J Cell Biochem. 2014 Jan;115(1):141-50. doi: 10.1002/jcb.24641.
Receptor tyrosine kinases (RTKs) expression and the growth factor such as platelet-derived growth factor (PDGF) and their receptors have been considered relevant in the process of angiogenesis and dissemination in esophageal squamous cell carcinoma (ESCC). Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) downstream of RTK signaling was a critical regulator of PDGF receptors (PDGFR) stability. The aim of the present study was to examine the expression of SGTA and to elucidate its clinicopathologic significance in ESCC. Immunohistochemistry and western blot analysis were performed for SGTA in ESCC samples. SGTA was up-regulated in ESCC as compared with the adjacent normal tissue. High expression of SGTA was associated with tumor grade (P < 0.01), and SGTA was positively correlated with proliferation marker Ki-67 (P < 0.05). Univariate analysis showed that SGTA expression did has a remarkable prediction for poor prognosis (P = 0.016). Knockdown or overexpression of SGTA affected ESCC cells proliferation and cell cycle. Additionally, after ESCC cells silenced for SGTA were treated with cisplatin (an anti-ESCC agent), the cell growth was down-regulated. These findings suggested that SGTA was involved in the pathogenesis of ESCC and might indicate a poor prognosis for ESCC patients.
受体酪氨酸激酶（RTKs）的表达以及血小板衍生生长因子（PDGF）等生长因子及其受体，在食管鳞状细胞癌（ESCC）的血管生成和扩散过程中被认为具有相关性。RTK信号下游的富含谷氨酰胺的小四肽重复序列蛋白α（SGTA）是血小板衍生生长因子受体（PDGFR）稳定性的关键调节因子。本研究的目的是检测SGTA的表达，并阐明其在ESCC中的临床病理意义。对ESCC样本进行了SGTA的免疫组织化学和蛋白质印迹分析。与相邻正常组织相比，ESCC中SGTA表达上调。SGTA高表达与肿瘤分级相关（P < 0.01），且SGTA与增殖标志物Ki-67呈正相关（P < 0.05）。单因素分析表明，SGTA表达对预后不良具有显著预测价值（P = 0.016）。敲低或过表达SGTA会影响ESCC细胞的增殖和细胞周期。此外，在用顺铂（一种抗ESCC药物）处理沉默了SGTA的ESCC细胞后，细胞生长受到抑制。这些发现提示SGTA参与了ESCC的发病机制，可能预示ESCC患者预后不良。
