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长春花冠瘿瘤中促进烟草细胞分裂的内源性因子的结构特征。

The structural characterization of endogenous factors from Vinca rosea crown gall tumors that promote cell division of tobacco cells.

作者信息

Lynn D G, Chen R H, Manning K S, Wood H N

出版信息

Proc Natl Acad Sci U S A. 1987 Feb;84(3):615-9. doi: 10.1073/pnas.84.3.615.

Abstract

The ability of two compounds, a cytokinin and an auxin, to stimulate tobacco cell growth and differentiation has been known for greater than 30 years, but the molecular mechanism of this activation is still unknown. Previous reports of factors endogenous in crown gall tumors of Vinca rosea that could replace the cytokinin requirement in tobacco cell culture has motivated an investigation of these tissues. The optimization of a reverse-phase isolation scheme has led to the purification of sufficient material to allow for the identification of six different related compounds. The structures of two of these compounds have been assigned as a set of epimeric dehydrodiconiferyl alcohol beta-D-glycosides. The structure of these compounds suggests that they would most likely be derived from the plant cell wall.

摘要

细胞分裂素和生长素这两种化合物刺激烟草细胞生长和分化的能力已为人所知超过30年,但这种激活的分子机制仍然未知。之前有关长春花冠瘿瘤中内源性因子可替代烟草细胞培养中细胞分裂素需求的报道促使人们对这些组织展开研究。反相分离方案的优化已使足够量的物质得以纯化,从而能够鉴定出六种不同的相关化合物。其中两种化合物的结构已确定为一组差向异构的脱氢二松柏醇β-D-糖苷。这些化合物的结构表明它们很可能源自植物细胞壁。

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