Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, Marseille, France.
J Exp Med. 2013 Aug 26;210(9):1657-64. doi: 10.1084/jem.20130403. Epub 2013 Aug 12.
Langerhans cells (LCs) constitute a network of immune sentinels in the skin epidermis that is seeded during embryogenesis. Whereas the development of LCs has been extensively studied, much less is known about the homeostatic renewal of adult LCs in "nonmanipulated" animals. Here, we present a new multicolor fluorescent fate mapping system and quantification approach to investigate adult LC homeostasis. This novel approach enables us to propose and provide evidence for a model in which the adult epidermal LC network is not formed by mature coequal LCs endowed with proliferative capabilities, but rather constituted by adjacent proliferative units composed of "dividing" LCs and their terminally differentiated daughter cells. Altogether, our results demonstrate the general utility of our novel fate-mapping system to follow cell population dynamics in vivo and to establish an alternative model for LC homeostasis.
郎格汉斯细胞（LCs）构成了皮肤表皮中免疫哨兵的网络，这些细胞在胚胎发生期间定植。虽然 LCs 的发育已经得到了广泛的研究，但对于“未受干扰”动物中成年 LCs 的稳态更新知之甚少。在这里，我们提出了一种新的多色荧光示踪系统和定量方法来研究成年 LC 的稳态。这种新方法使我们能够提出并提供证据，证明成年表皮 LC 网络不是由具有增殖能力的成熟 LC 组成的，而是由相邻的增殖单元组成，这些增殖单元由“分裂”的 LC 和它们终末分化的子细胞组成。总的来说，我们的结果表明，我们的新型示踪系统可用于在体内跟踪细胞群体动力学，并建立 LC 稳态的替代模型。
